槟榔中槟榔碱含量的微流控芯片非接触电导法测定  

Determination of Arecoline in Areca Catechu by Microfluidic Chip with Contactless Conductivity Detection

作　　者：杨秀娟[1] 陈缵光[2] 刘艳清[3] 

机构地区：[1]南方医科大学珠江医院,广东广州510280 [2]中山大学药学院,广东广州510006 [3]广州市第八人民医院,广东广州510060

出　　处：《时珍国医国药》2015年第6期1327-1329,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81302738)

摘　　要：目的建立微流控芯片非接触电导法测定槟榔饮片中槟榔碱含量。方法在高电场强度下,利用芯片上微通道实现待测组分的分离,以非接触电导检测器进行检测;研究电泳介质、进样时间和分离电压等因素对分离检测的影响。结果优化条件为：缓冲溶液2 mmol·L-1醋酸＋2 mmol·L-1醋酸钠（p H=4.7）;进样时间10 s;分离电压2.5 k V。槟榔碱的线性范围为10-200μg·ml-1（r=0.9998）,RSD为1.7%,加标回收率为98.6%-99.7%。结论该方法简便快速,重现性好,为槟榔的质量控制提供了一种新方法。Objective To establish the method for the content determination of arecoline in areca catechu by microfluidic chip with contactless conductivity detection. Methods Microfluidic chip with contactless conductivity detection was adopted. Arecoline was rapidly separated by microfluidic chip and detected with contactless conductivity detection under high electric field. The electrophoretic parameters,such as the variety and concentration of buffer solution and additive,injection time and separation voltage,etc.,were analyzed. Results In optimal conditions,using 2 mmol·L- 1HAc and 2 mmol·L- 1Na Ac（ p H = 4. 7） as buffer solution without additive at the separation voltage of 2. 5 k V and injection time of 10 s,arecoline was separated and detected within20 s. The linear range of arecoline ranged from 10 to 200 μg·ml- 1（ r = 0. 9998） with the detection limit of 2. 0 μg·ml- 1（ S/N = 3） with RSD of 1. 7% and the recoveries from 98. 6% to 99. 7%. Conclusion The method is simple,rapid and well reproducible,and it could be an alternative to the traditional methods in the quality control of areca catechu.

关 键 词：微流控芯片 非接触电导检测 槟榔碱 槟榔 

分 类 号：R284.1[医药卫生—中药学]