粗叶悬钩子叶的聚酰胺薄层色谱-串联质谱分析  

Analysis of compounds in Rubus aleaefolius Poir. leaves by polyamide TLC-MS/MS

作　　者：郭依俐[1,2] 唐青涛 张芳荣[4] 赵宏伟 刘韬[2] 赵志敏[1] 

机构地区：[1]中山大学药学院天然药物与中药研究所,广东广州510006 [2]中山大学肿瘤防治中心药学部,广东广州510006 [3]无限极中国有限公司,广东新会529156 [4]台湾中山大学,中国台湾高雄80402

出　　处：《时珍国医国药》2015年第6期1390-1392,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目青年科学基金项目(No.81102782);中山大学山海论坛合作项目专项基金

摘　　要：目的快速分析粗叶悬钩子叶中成分。方法粗叶悬钩子叶甲醇提取物用聚酰胺薄层色谱(TLC)分离后,将薄层板上的斑点依次用串联质谱(MS/MS)分析,通过与文献数据和部分对照品的负离子模式下的一级和二级质谱信息比较,推测化合物的结构。结果推测了11个化合物的可能结构,包括1个咖啡酰奎宁酸,6个三萜化合物和4个黄酮苷。结论实验结果为粗叶悬钩子叶活性成分的快速鉴定奠定了基础。Objective To analyze compounds in Rubus aleaefolius Poir. leaves. Methods The Rubus aleaefolius Poir. leaves methanol extract was separated by polyamide TLC,and then the spots on TLC chromatogram were analyzed by MS / MS in turn.The structures of compounds were tentatively identified by comparison of their mass spectra with those of some authentic samples and literatures. Results Eleven compounds were tentatively identified,including one caffeoylquinic acid,six triterpenoids and four flavonoids. Conclusion This result set up the foundation for rapid characterization of active compounds in Rubus aleaefolius Poir. leaves.

关 键 词：粗叶悬钩子叶 成分 聚酰胺薄层色谱 串联质谱 

分 类 号：R284.2[医药卫生—中药学]