肾阳虚模型对甘氨酸受体mRNA表达的影响  

Influence on Expression of Glycine receptor mRNA in Kidney-Yang deficiency Syndrome

作　　者：林海雄[1] 王晓彤[1] 张韧[2] 

机构地区：[1]广州中医药大学第一临床医学院,广东广州510405 [2]广州中医药大学基础医学院,广东广州510006

出　　处：《时珍国医国药》2015年第6期1518-1520,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81102714);广东省大学生创新实验项目(No.1057212011);广东省大学生创新创业训练计划项目(No.201410572065;201410572077)

摘　　要：目的探讨肾阳虚简易有效的建模方法和对甘氨酸受体(GlycR)mRNA表达的影响。方法用腺嘌呤皮下注射构建肾阳虚大鼠模型,观察大鼠行为变化,测定其自主活动度、脏器指数和体重,做肾脏切片,并运用Real-time RT-PCR测定中枢神经GlycR mRNA的相对表达量。结果造模后,大鼠肾脏、睾丸指数增大;肾皮质固缩,与髓质界限模糊,有灰褐色沉积物;与正常组相比,大鼠自主活动度降低(P<0.05),GlycR mRNA的相对表达明显增高(P<0.05)。结论用腺嘌呤建立肾阳虚大鼠模型方法可靠方便,可能会增强GlycR mRNA的表达。Objective To explore a simple and effective method of Kidney- Yang deficiency in rats,and the expression of Glycine receptor（ GlycR） mRNA. Methods Rats were injected subcutaneously with adenine to build Kidney- Yang deficiency rats model. The behavior,kidney,testis mass,kidney biopsy and body weight of Kidney- Yang deficiency rats were measured and compared to rats without any treatments（ negative control group）. Real- time RT- PCR was employed to measure GlycR mRNA expression. Results Kidney index and testis index increased in Kidney- Yang deficiency rats. Renal cortical shrinkage and have gray- brown sediment,the border of cortex and medulla is unclear. Compared to Kidney- Yang deficiency rats,locomotor activity in low- dose group of rats significantly reduced（ P 0. 05）,and GABAa mRNA expression significantly increased（ P 0.05）. Compared to the negative control group,GlycR mRNA of rats was significantly increased（ P 0. 05）. Conclusion It is available for using adenine to build a Kidney- Yang deficiency rat model and Kidney- Yang deficiency rats have increased glycine receptor expression.

关 键 词：肾阳虚 Glyc R MRNA 

分 类 号：R2-03[医药卫生—中医学]