南疆43份引进及本地苜蓿种质资源同工酶分析  

Analysis of 43 Introduced and Local Alfalfa Germplasm Resources of Southern Xinjiang Using Isozyme Markers

作　　者：陈立强[1] 王连群[1] 马春晖[2] 颜静[1] 周海宁[1] 陈天峰[1] 

机构地区：[1]塔里木大学动物科学学院/塔里木畜牧科技兵团重点实验室,新疆阿拉尔843300 [2]石河子大学动物科技学院,新疆石河子832000

出　　处：《新疆农业科学》2015年第5期913-920,共8页Xinjiang Agricultural Sciences

基　　金：国家牧草产业技术体系(CARS-35);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201103);新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201405);"十二五"国家科技支撑计划;"新疆绿洲区优质牧草选育及生产利用技术集成与示范"(2011BAD17B05-2)

摘　　要：【目的】对南疆地区引进及本地苜蓿种质资源的亲缘关系进行分析,为地区苜蓿新品种培育提供科学依据。【方法】以43份引进苜蓿品种、新疆大叶和拜城野生紫花苜蓿为材料,用过氧化物酶、淀粉酶、过氧化氢酶和酯酶电泳技术对其亲缘关系进行分析。【结果】4种同工酶在43份苜蓿材料中共检测到38条稳定酶带,过氧化物酶、淀粉酶、过氧化氢酶和酯酶的多态位点百分率分别为91.67%、66.67%、66.67%和75.00%。材料间的相似系数0.579-0.974,平均为0.798。43份材料在相似系数0.725处可聚为3类,拜城野生紫花苜蓿和河西紫花苜蓿分别被单独聚为类。捷克、拜城野生紫花苜蓿、河西、赛特、格林、渭南、秘鲁与大部分材料的亲缘关系较远。【结论】43份苜蓿材料具有一定水平的遗传差异,部分材料具有遗传特异性。【Objective】The purpose of this study is to investigate the genetic relationship of introduced and local alfalfa germplasm resources of Southern Xinjiang and provide scientific basis for future alfalfa breeding programs.【Method】The genetic relationship of 41 introduced alfalfa cultivars,Xinjiangdaye and Baicheng wild alfalfa were analyzed by using peroxidase,amylase,catalase and esterase electrophoresis.【Result】The results showed that 4 isozymes generated a total of 38 stable bands across 43 alfalfa accessions,and the percentage of polymorphic bands for peroxidase,amylase,catalase and esterase were 91. 67%,66. 67%,66. 67% and75. 00%,respectively. The similarity coefficient among alfalfa accessions ranged from 0. 579 to 0. 974 with an average of 0. 798. The accessions could be classified into 3 groups at the similarity coefficient value of 0. 725 based on cluster analysis,among which baicheng wild alfalfa and hexi were clustered into 2 independent groups. According to principal component analysis,czech,baicheng wild alfalfa,hexi,saite,grimm,weinan andperu had relatively distant genetic relationships with most accessions.【Conclusion】There were a certain genetic differences among 43 alfalfa accessions,and some accessions had genetic specificity.

关 键 词：苜蓿 同工酶 亲缘关系 种质资源 

分 类 号：S551.7[农业科学—作物学]