雷公藤多苷片联合窄谱紫外光子对寻常银屑病患者T淋巴细胞亚群和细胞因子的影响分析  

Effects of tripterygium glycosides tablet combined with narrow spectrum ultraviolet photon on T lymphocyte subsets and cytokines in patients with psoriasis vulgaris

作　　者：李红林[1] 熊霞[1] 

机构地区：[1]四川泸州市泸州医学院附属医院皮肤科,四川泸州646000

出　　处：《现代预防医学》2015年第13期2415-2418,共4页Modern Preventive Medicine

基　　金：凋亡相关蛋白Livin和Caspase-3在日光性角化病发病机制中的作用研究,泸州市科技局市级项目(编号12104)

摘　　要：目的探讨了雷公藤多苷片联合311 nm窄谱紫外光子对寻常银屑病患者T淋巴细胞亚群和细胞因子的影响。方法选取2011年6月-2013年6月来本院进行治疗的寻常银屑病患者84例,随机分为A、B两组,每组42例,A组采用雷公藤多苷片治疗;B组在A组基础上给予311 nm窄谱紫外光子联合治疗;同时选取30例健康体检者为正常对照组。抽取两组患者治疗前后及对照组外周血,分离外周血淋巴细胞与血清,采用流式分析和和ELISA法测定各组患者治疗前后淋巴细胞亚群变化情况以及血清中IFN-γ、IL-2以及s IL-2R水平的影响。结果与对照组相比,两组患者治疗前外周血CD3+、CD4+细胞比例以及CD4+/CD8+比值均下降,而CD8+细胞比例则升高;经过治疗后,A组患者外周血CD3+、CD4+细胞比例以及CD4+/CD8+比值有所升高,但上升幅度不显著(P>0.05),CD8+细胞比例无变化;而B组CD3+、CD4+细胞比例以及CD4+/CD8+比值则升高幅度显著,CD8+细胞比例则显著降低(P<0.05),各项指标接近正常水平;治疗前A、B两组患者血清中IFN-γ、IL-2以及s IL-2R水平显著高于对照组(P<0.05),经过治疗后,A、B两组患者血清中IL-2以及s IL-2R水平均显著下降,且B组下降幅度更为显著(P<0.05);B组患者血清中IFN-γ水平下降幅度显著(P<0.05),A组IFN-γ水平则无显著性变化(P>0.05)。结论雷公藤多苷片联合320nm窄谱紫外光子治疗能够显著改善寻常银屑病患者T淋巴细胞亚群比例以及细胞因子的表达,疗效显著,明显优于单一疗法。Objective To investigate the effects of tripterygium glycosides tablet combined with 311 nm narrow spectrum ultraviolet photon on T lymphocyte subsets and cytokines in patients with psoriasis vulgaris. Methods 84 patients with psoriasis vulgaris from June 2011 to June 2013 in our hospital were randomly divided into groups A and B. Patients of group A were treated with Tripterygium wilfordii. Patients of group B received 311 nm narrow spectrum ultraviolet photon therapy based on group A. At the same time, we selected 30 cases of healthy people as the control group. Lymphocyte subsets of peripheral blood in three groups were analyzed using flow cytometry assay before and after treatment. Serum levels of IFN-γ, IL-2 and s IL-2R was analyzed using ELISA. Results Compared with control group, CD3＋cells, CD4＋cells and CD4＋/CD8＋ratio of peripheral blood in group A decreased, but the proportion of CD8＋cells increased. After treatment, CD3＋cells, CD4＋cells and CD4＋/CD8＋ratio increased, but the increase was not significant（P〈0.05）, CD8＋ cell had no change. While in B group, CD3＋cells, CD4＋cells and CD4＋/CD8＋ratio increased significantly and CD8＋ cells decreased significantly（P〈0.05）, each index was close to the normal level. Before treatment,the serum levels of IFN-γ, IL-2 and s IL-2R levels in groups A and B were significantly higher than those in the control group（P〈0.05）. After treatment, IL-2 and s IL-2R levels decreased significantly, and the levels in group B significantly decreased more（P〈0.05）; IFN-γ level in group B decreased significantly compared with group A（P〈0.05） and the levels of IFN-γ in group A had no significant changes（P〉0.05）. Conclusion Tripterygium glycosides tablet combined with 311 nm narrow spectrum ultraviolet photon can significantly ameliorate the proportion of T lymphocyte subsets and the express of cytokines in patients with psoriasis vulgaris than the single therapy.

关 键 词：雷公藤多苷片 311 nm窄谱紫外光子 T淋巴细胞亚群 IFN-Γ IL-2 s IL-2R 

分 类 号：R113[医药卫生—公共卫生与预防医学]