胰岛素自微乳剂肠道给药的降血糖效果考察  

Study on the Hypoglycemic Effect of Insulin Self-microemulsion for Parenteral Administration

作　　者：黄惠燕[1] 梁丽梅[1] 余芬[1] 吴美琴[1] 张晓娟[1] 黄小琼[2] 

机构地区：[1]广东省人民医院/广东省医学科学院,广州510120 [2]广东医学实验动物中心,广东佛山528248

出　　处：《中国药房》2015年第19期2638-2640,共3页China Pharmacy

基　　金：广东省科技计划基金(No.2011B031800010)

摘　　要：目的：研究胰岛素自微乳剂肠道给药在1型糖尿病模型大鼠体内的降血糖效果。方法：取大鼠ip给予链脲佐菌素（50mg/kg）复制1型糖尿病模型,将模型大鼠随机分为模型（生理盐水）组、阳性对照（胰岛素注射液2.25 u/kg）组和微乳剂低、中、高剂量组（胰岛素自微乳剂4.5、9、18 u/kg）;另取正常大鼠10只为假手术（生理盐水）组。均麻醉手术,除阳性对照组大鼠ip给药外,其余大鼠肠道给药,于给药前和给药后15~600 min检测各组大鼠血糖水平。另分别对正常对照组、模型未给糖组、模型给糖组和微乳剂（胰岛素自微乳剂9 u/kg）组大鼠进行糖耐量实验（除模型未给糖组大鼠不给糖外其余大鼠均给糖）。结果：与假手术组比较,模型组大鼠给药后0~240 min内的血糖值均增加,差异具有统计学意义（P〈0.05）。与模型组比较,阳性对照组大鼠给药15 min后有降血糖趋势,给药后30~480 min内的血糖值均减小,其中降血糖峰值为36%、峰值时间为30 min;微乳剂低、中、高剂量组大鼠给药30 min后有降血糖趋势,给药后45~360 min内的血糖值均减小,其中降血糖峰值为18%~21%、峰值时间为90~120min,以上差异具有统计学意义（P〈0.01或P〈0.05）。糖耐量实验中除模型未给糖组外其余各组大鼠均有血糖吸收峰,且微乳剂组大鼠血糖达峰值后迅速降低。结论：胰岛素自微乳剂能明显降低1型糖尿病模型大鼠的血糖。OBJECTIVE：To study the hypoglycemic effects of Insulin self-microemulsion for parenteral administration on model rats with type 1 diabetes in vivo.METHODS：Rats were treated with streptozotocin（50 mg/kg）to reproduce model with type 1 diabetes,ip.The model rats were randomly divided into model group（normal saline）,positive control group（Insulin injection2.25 u/kg）and self-microemulsion low,medium and high dose groups（Insulin self-microemulsion 4.5,9 and 18 u/kg）;and 10 normal rats were involved in sham-operation group（normal saline）.Anesthesia and operation were conducted for all rats.Positive control group was administrated,ip;other rats were parenterally administrated.The blood glucose levels in groups were detected before and after 15-600 min administration.Glucose tolerance test was conducted for the rats in normal control group,model group without glucose,model group with glucose and microemulsion group（Insulin self-microemulsion 9 u/kg）.All group were given glucose except model group without glucose.RESULTS：Compared with sham-operation group,the blood glucose levels in model group within 0-240 min were increased,with significant difference（P〈0.05）.Compared with model group,there was hypoglycemic trend in positive control group after 15 min,the blood glucose levels within 30-480 min were decreased,the hypoglycemic peak was 36%,and the peak time was 30 min;there was also hypoglycemic trend in microemulsion low,medium and high dose groups after 30 min,the blood glucose levels within 45-360 min were decreased,hypoglycemic peak was 18%-21%,and the peak time was 90-120 min,with significant differences（P〈0.01 or P〈0.05）.All rats had glucose absorption peak except for model group without glucose in glucose tolerance test,and glucose of rats in microemulsion group reached its peak and then quickly decreased.CONCLUSIONS：Insulin self-microemulsion can obviously reduce the blood glucose of model rats with type 1 diabetes.

关 键 词：胰岛素 自微乳剂 糖尿病 大鼠 药效学 

分 类 号：R965[医药卫生—药理学]