胃得安片对应激性胃溃疡模型大鼠的保护作用  

Protective Effect of Weidean Tablets on Rats with Stress-induced Gastric Ulceration

作　　者：毕云生[1] 李允江[2] 杨文超[2] 路其康 于燕莉[1] 

机构地区：[1]济南军区总医院药剂科,济南250031 [2]山东中医药大学药学院,济南250355

出　　处：《中国药房》2015年第19期2654-2657,共4页China Pharmacy

摘　　要：目的:研究胃得安片对应激性胃溃疡模型大鼠的保护作用。方法:采用空腹、水浸拘束法复制大鼠胃溃疡模型。60只大鼠随机均分为正常对照(等容氯化钠注射液)组、模型(等容氯化钠注射液)组、雷尼替丁(0.015 g/kg)组与胃得安片高、中、低剂量(1.70、0.87、0.43 g/kg)组。观察大鼠胃大体情况,测定大鼠胃黏膜损伤指数、血清一氧化氮(NO)含量、胃组织超氧化物歧化酶(SOD)、诱导型一氧化氮合酶(i NOS)活性与丙二醛(MDA)、前列腺素E2(PGE2)含量,光学显微镜下观察大鼠胃组织病理变化。结果:与正常对照组比较,模型组大鼠胃黏膜损伤指数升高,血清NO含量减少,胃组织SOD活性减弱、i NOS活性增强、MDA和PGE2含量增加,差异有统计学意义(P<0.01或P<0.05);大鼠胃内出现暗红物质,黏膜细胞出现严重变形坏死,并出现多发性大面积浅表性溃疡。与模型组比较,胃得安片高、中、低剂量组大鼠胃黏膜损伤指数降低,血清NO含量增加,胃组织SOD活性增强、i NOS活性减弱、MDA含量减少、PGE2含量增加,差异有统计学意义(P<0.01或P<0.05);大鼠胃大体情况与组织病理情况有所改善。结论:胃得安片对应激性刺激导致的胃溃疡大鼠具有一定保护作用,其机制与改善大鼠血清抗氧化指标水平有关。OBJECTIVE：To study the protective effect of Weidean tablets on rats with stress-induced gastric ulceration.METHODS：Water immersion restraint stress method and empty stomach were employed to establish rat models with gastric ulceration.60 SD rats were equally randomized into normal control group（isometric sodium chloride injection）,model group（isometric sodium chloride injection）,ranitidine group（0.015 g/kg）,and groups of high,medium and low doses（1.70,0.87 and 0.43 g/kg）of Weidean tablets.General situation of stomach was observed.The gastric mucosal injury index,the NO content in serum,and the activities of SOD and i NOS and the contents of MDA and PGE2 in the gastric tissue were detected for rats.Pathomorphological observation of rats＇ gastric tissues was conducted under the microscope.RESULTS：Compared with normal control group,the rats in the model group had higher gastric mucosal injury index,lower content of NO in serum,and weaker activity of SOD,stronger activity of i NOS,higher content of MDA and PGE2,in the gastric tissue.There was statistical significance（P0.01 or P0.05）.Dark matter,severe deformation and necrosis of gastric mucosal cells in rats were noted,as well as multiple large-area superficial ulcers.Compared with the model group,the rats in groups of high,medium and low doses of Weidean tablets had lower gastric mucosal injury index,higher content of NO in serum,and stronger activity of SOD,weaker activity of i NOS,lower content of MDA and higher content of PGE2,in the gastric tissue.There was statistical significance（P0.01 or P0.05）.General situation of stomach and the pathomorphological condition of rats＇ gastric tissues were improved.CONCLUSIONS：Weidean tablets has protective effect to some extent on rats with stress-induced gastric ulceration,the mechanism is related to the improvement of serum levels of anti-oxidation index in rats.

关 键 词：胃得安片 胃溃疡 超氧化物歧化酶 丙二醛 前列腺素E2 大鼠 

分 类 号：R285[医药卫生—中药学] R97[医药卫生—中医学]