旋覆归连方醇提液镇痛抗感染作用的实验研究  

Experimental Research on Analgesic and Anti- inflammatory Effect of Xuanfuguilian Prescription Extracted by Ethanol

作　　者：高小玲[1] 崔姗姗[1] 汪保英[1] 王慧慧[1] 陈玉龙[1] 

机构地区：[1]河南中医学院,河南省郑州市450008

出　　处：《中国全科医学》2015年第18期2201-2204,共4页Chinese General Practice

基　　金：国家自然科学基金面上项目(81173177);河南省教育厅自然科学基金项目(15B360003);河南中医学院博士科研启动基金(2010BSJJ-12);河南中医学院科研苗圃工程项目(MP2012-19)

摘　　要：目的探讨旋覆归连方醇提液镇痛及抗感染作用。方法选取SPF级昆明小鼠240只,采用随机数字表法分为5组,空白对照组、阿司匹林组(阿司匹林溶液0.2 g·kg-1·d-1,0.01 g/ml)或地塞米松组(地塞米松0.02 g·kg-1·d-1)、旋覆归连方低剂量组(旋覆归连方醇提液0.45 g/kg)、旋覆归连方中剂量组(旋覆归连方醇提液0.90 g/kg)、旋覆归连方高剂量组(旋覆归连方醇提液1.80 g/kg)。比较各组小鼠热板致痛作用(记录小鼠痛阈)、冰乙酸致痛作用(记录小鼠扭体次数)、二甲苯致炎作用(记录小鼠耳郭肿胀度)及肉芽肿组织净质量。结果痛阈治疗方法与时间无交互作用(P>0.05);组间痛阈比较,差异无统计学意义(P>0.05);不同时间痛阈比较,差异有统计学意义(P<0.05)。与空白对照组比较,阿司匹林组、旋覆归连方低剂量组、旋覆归连方中剂量组、旋覆归连方高剂量组小鼠扭体次数减少(P<0.05);与阿司匹林组比较,旋覆归连方低剂量组、旋覆归连方中剂量组、旋覆归连方高剂量组小鼠扭体次数增多(P<0.05);与旋覆归连方中剂量组比较,旋覆归连方高剂量组小鼠扭体次数减少(P<0.05)。地塞米松组小鼠耳郭肿胀度较空白对照组减轻(P<0.05);空白对照组、旋覆归连方低剂量组、旋覆归连方中剂量组、旋覆归连方高剂量组小鼠耳郭肿胀度比较,差异无统计学意义(P>0.05)。与空白对照组比较,地塞米松组、旋覆归连方低剂量组、旋覆归连方中剂量组、旋覆归连方高剂量组小鼠肉芽肿组织净质量减少(P<0.05);地塞米松组、旋覆归连方低剂量组、旋覆归连方中剂量组、旋覆归连方高剂量组小鼠肉芽肿组织净质量比较,差异无统计学意义(P>0.05)。结论旋覆归连方醇提液能减少冰乙酸所致小鼠扭体次数,减轻包埋棉球所致的慢性肉芽肿。Objective To investigate the analgesic and anti - inflammatory effect of Xuanfuguilian prescription extracted by ethanol. Methods SPF level Kunming mice were divided into five groups by random number table method：blank control group,aspirin group（aspirin solution 0. 2 g·kg - 1 ·d - 1 ,0. 01 g/ ml）or dexamethasone group（dexamethasone 0. 02 g·kg - 1 · d - 1 ）,low dose Xuanfuguilian prescription group（ Xuanfuguilian prescription extracted by ethanol 0. 45 g/ kg）, middle dose Xuanfuguilian prescription group（ Xuanfuguilian prescription extracted by ethanol 0. 90 g/ kg）,and high dose Xuanfuguilian prescription group （ Xuanfuguilian prescription extracted by ethanol 1. 80 g/ kg ） . The analgesic effects of Xuanfuguilian prescription extracted by ethanol were evaluated by hot plate - induced pain experiment and glacial acetic acid -induced pain assay. The anti - inflammatory effects were evaluated by xylene - induced imflammation experiment and net content of cotton ball - induced granuloma. Results There was no interaction between the treatment method and treatment time（ P 〉0. 05）;there was no significant difference in the pain threshold among different groups（ P 〉 0. 05）;there were significant differences in the pain threshold among different treatment times（P 0. 05 ） . The net content of granuloma of mice in dexamethasone group,low dose Xuanfuguilian prescription group,middle dose Xuanfuguilian prescription group,and high dose Xuanfuguilian prescription group was significantly lower than that of mice in blank control group,respectively（P 0. 05） . Conclusion Xuanfuguilian prescription extracted by ethanol can reduce glacial acetic acid - induced writhing times of mice,alleviate cotton ball - induced granuloma.

关 键 词：抗炎药(中药) 镇痛 旋覆归连方 

分 类 号：R28[医药卫生—中药学]