中国小麦品种兰天9号慢叶锈性QTL分析  

QTL Mapping for Adult-Plant Resistance to Leaf Rust in Chinese Wheat Cultivar Lantian9

作　　者：韩柳莎[1] 王佳真[1] 师令智[1] 朱琳[1] 李星[1] 刘大群[1] 

机构地区：[1]河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心,河北保定071001

出　　处：《中国农业科学》2015年第12期2346-2353,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(国际(地区)合作与交流项目;31361140367);河北省教育厅优秀青年项目(YQ2013024);高等学校博士学科点专项科研基金联合资助课题(20131302120004)

摘　　要：【目的】由小麦叶锈菌(Puccinia triticina)引起的小麦叶锈病是影响小麦稳产、高产的一种重要真菌病害。目前防治小麦叶锈病最经济、安全、有效的方法是种植抗病品种。中国小麦品种兰天9号苗期对大多数叶锈菌小种表现感病,成株期对小麦叶锈菌则表现为明显的慢锈性。研究旨在分析中国小麦品种兰天9号的成株抗叶锈性,发掘其中含有的QTL,并利用分子标记进行定位,为小麦分子育种提供理论基础。【方法】利用抗病亲本兰天9号和感病亲本辉县红杂交获得到197个家系的F2:3群体,2011—2014年连续3年在河北保定种植,并利用3个叶锈菌生理小种混合菌种(THTT、THTS、THTQ)进行田间接菌,小麦成株期调查最终发病严重度,获得表型数据。利用1 232对SSR标记对兰天9号、辉县红以及F2:3群体进行基因检测,获得基因型数据。结合表型数据和基因型数据,利用Map Manager QTXb20创建连锁图、QTL Icimapping 3.2软件进行抗叶锈病QTL分析。【结果】检测到5个QTL,其中位于2B染色体上的QTL暂命名为QLr.hbau-2BS,在连续两年的数据结果中都被检测到,解释的遗传变异分别为6.0%和9.1%;标记区间分别为Xbarc55-Xgwm148和Xgwm429-Xwmc154;LOD值分别为2.6和3.46;加性效应分别为-6.1和-8.7;显性效应分别为3.03和3.4。1B染色体上1个QTL暂命名为QLr.hbau-1BL.2,连续两年被检测到,解释的遗传变异分别为7.7%和10.7%;标记区间为Xwmc766—Xbarc269;LOD值分别为2.5和3.1;加性效应分别为-1.0和-1.1;显性效应分别为-13.0和-14.9。其他3个QTL只在一个年份被检测到,1B染色体上暂命名为QLr.hbau-1BL.1、4B上暂命名为QLr.hbau-4BS、3A上暂命名为QLr.hbau-3A,均在2011—2012年度检测到,解释的遗传变异分别为11.7%、8.5%、5.6%;标记区间分别为Xbarc80—Xwmc728、Xgwm495—Xwmc652和Xgwm161—Xbarc86;LOD值分别为5.1、4.0和2.8;加性效应分别为6.5、-5.5和-3.1;显性效应分别为-6.5、6.2和【Objective】Wheat leaf rust, caused by Puccinia triticina, is a devastating disease that can cause severe yield loss. The use of resistant cultivars is the most economical and effective method to reduce damages caused by wheat leaf rust. Wheat line Lantian 9 is susceptible to most of the Chinese current leaf rust pathotypes at seedling stage, but showed high resistance at adult stage. The objectives of this study are to analyze the leaf rust resistance of Chinese wheat cultivar Lantian 9 in adult-plant and locate the QTL contained in Lantian 9 by using molecular markers, and to provide a theoretical basis for molecular breeding in wheat. 【Method】Total 197 F2：3 lines derived from Lantian 9/Huixian Hong cross were used to map leaf rust QTL. The population was inoculated by three pathotypes, and then phenotyped for FDS in the field during 2011-2014 cropping seasons. A total of 1 232 SSR markers were used to detect resistant parent Lantian 9, susceptible parent Huixian Hong and F2：3 lines, to get the genotypic data. Phenotypic and genotypic data were combined to map adult plant resistance QTL in Lantian 9 and Huixian Hong using QTL softwares Map Manager QTXb20 and Icimapping 3.2.【Result】Five QTLs were detected in the population. The QTL located on chromosome 2B was stably identified in two cropping seasons, tentatively designated QLr.hbau-2BS, explained 6.0% and 9.1% of the phenotypic variations in two years, respectively. QLr.hbau-2BS was located in the marker interval Xbarc55-Xgwm148 and Xgwm429-Xwmc154. The logarithm of odds（LOD） threshold was 2.6 and 3.46. The additive effect was-6.1 and-8.7. The dominate effect was 3.03 and 3.4, respectively. The QTL located on chromosome 1B was stably identified in two cropping seasons, tentatively designated QLr.hbau-1BL.2, explained 7.7% and 10.7% of the phenotypic variations in two years, respectively. QLr.hbau-1BL.2 was located in the marker interval Xwmc766-Xbarc269. The LOD threshold was 2.5 and 3.1. The additive effect was-1.0 and-1.1. The dominate eff

关 键 词：小麦 叶锈病 成株抗病基因 遗传分析 QTL作图 

分 类 号：S512.1[农业科学—作物学]