UV-A诱导大豆芽苗菜下胚轴中花青苷积累的分子机理  

The Molecular Mechanism of UV-A Induced Anthocyanin Accumulation in the Hypocotyls of Soybean Sprouts

作　　者：戚楠楠[1] 张晓燕[1] 苏娜娜[1] 邬奇[1] 耿殿祥 齐学会 魏圣军 崔瑾[1] 

机构地区：[1]南京农业大学生命科学学院,南京210095

出　　处：《中国农业科学》2015年第12期2408-2416,共9页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31171998);国家基础科学人才培养基金(J1310015)

摘　　要：【目的】研究长波紫外光(UV-A)、白光(W)和蓝光(B)对大豆芽苗菜下胚轴中花青苷含量、花青苷合成相关酶活性、花青苷合成途径相关基因及光受体基因表达量的影响,以探明UV-A诱导大豆芽苗菜下胚轴中花青苷生物合成的分子机理,为光质调控技术应用于大豆芽苗菜工业化生产提供理论依据。【方法】以大豆‘东农690’为试材,以黑暗培养为对照,连续的UV-A、白光(W)和蓝光(B)光照培养作为试验处理,在处理0 h、12h、24 h和36 h后各采样一次,分别测定花青苷含量,苯丙氨酸解氨酶(PAL)、查尔酮异构酶(CHI)以及类黄酮半乳糖苷转移酶(UFGT)活性,相关基因(PAL、CHS、CHI、DFR、ANS、UFGT、MYB75、CRY1、CRY2、UVR8)表达量。花青苷含量采用分光光度法测定,苯丙氨酸解氨酶(PAL)及查尔酮异构酶(CHI)活性采用分光光度法测定,类黄酮半乳糖苷转移酶(UFGT)活性采用超高效液相色谱法(UPLC)测定。材料总RNA采用Trizol试剂法提取,基因表达量采用q RT-PCR测定。【结果】黑暗培养下的大豆芽苗菜子叶为黄色,而白光(W)、蓝光(B)和UV-A培养下的大豆芽苗菜子叶为绿色。与黑暗培养及其他光质处理相比,UV-A显著提高大豆芽苗菜下胚轴中花青苷含量;随着处理时间的延长,花青苷积累逐渐增加。0 h处理下,大豆芽苗菜下胚轴中花青苷含量较低,约2 U·g-1FW。经36 h的UV-A处理,大豆芽苗菜下胚轴中花青苷含量达到最大值(43 U·g-1FW),显著高于黑暗及其他光照处理。0 h处理下,PAL和CHI酶活性较高。与黑暗培养及其他光质处理相比,24 h及36 h UV-A处理显著提高了PAL酶活性;12 h及24 h UV-A处理显著提高了UFGT酶活性。0 h处理下,不同处理间的花青苷合成相关基因表达均无差异。与黑暗培养及其他光质处理相比,UV-A处理36 h显著上调了MYB75、CRY1、CRY2及UVR8的表达,分别上调约12倍、30倍、6倍和2倍;UV-A处理12 h显著上调了花青苷合�【Objective】The objective of the experiment was to investigate the effect of UV-A, as well as white（W） and blue（B） lights on content of anthocyanin, activities of related enzymes, transcript levels of anthocyanin biosynthesis-related genes and light receptor genes. This study would find out the molecular mechanism about UV-A induces anthocyanin accumulation in the hypocotyls of soybean sprouts and provide a theoretical foundation for the application of light quality control technology in the industrialized production of soybean sprouts.【Method】The seedlings of soybean variety ‘Dongnong 690＇ were treated with continuous UV-A, white light（W） and blue light（B）. Samples were collected at 0, 12, 24 and 36 h after treatment, respectively. The anthocyanin content, the activities of PAL, CHI, and UFGT and the expression of related genes（PAL, CHS, CHI, DFR, ANS, UFGT, MYB75, CRY1, CRY2 and UVR8） were detected. The difference of anthocyanin content, related enzymes and genes expression under different light qualities in the hypocotyls of soybean sprouts was analyzed. The anthocyanin content was determined by spectrophotometric method, phenylalanine ammonia lyase（PAL） and chalcone isomerase（CHI） activities were measured by spectrophotometry, flavonoid galactosyl transferase（UFGT） activity by ultra performance liquid chromatography（UPLC） determination. Total RNA was extracted using the Trizol reagent. Gene expression was determined by q RT-PCR.【Result】Soybean sprouts grown in the dark showed yellow cotyledons while sprouts grown under white（W）, blue（B） and UV-A lights existed green cotyledons. Compared with the dark and other light quality cultures, UV-A treatment significantly improved the content of anthocyanin in the hypocotyls of soybean sprouts. As the illumination time goes on, anthocyanin accumulation was increased gradually. The anthocyanin content in the hypocotyls of soybean sprouts was low at 0 h, about 2 U·g-1 FW. The content of anthocyanin reached a max

关 键 词：大豆芽苗菜 花青苷 UV-A 分子机制 

分 类 号：S649[农业科学—蔬菜学]