硝普钠对保存期间悬浮红细胞变形性的作用研究  

The Effects of Sodium Nitroprussiate on Deformability of Suspended Red Blood Cells

作　　者：李海军[1] 

机构地区：[1]广元市中心医院检验科,广元628000

出　　处：《成都医学院学报》2015年第3期290-293,共4页Journal of Chengdu Medical College

摘　　要：目的探讨硝普钠对保存期间悬浮红细胞变形性的影响。方法来源于血站的50袋悬浮红细胞,200mL/袋,在保存期的第1、3、5、7、14、21、28、35天(D1、D3、D5、D7、D14、D21、D28、D35)共8个时间点,各取20mL,分成4管(5mL/管),其中,1管不做处理为对照组(A组),另外3管分别添加硝普钠,配置成终浓度为B组1μmol/L、C组5μmol/L和D组10μmol/L。检测4组的红细胞变形指数、聚集指数和刚性指数。结果与A组红细胞变形指数比较,B组和D组差异无统计学意义(P>0.05),C组明显降低(P<0.05);与A组聚集指数和刚性指数比较,B、C、D 3组之间差异无统计学意义(P>0.05)。结论浓度为5μmol/L的硝普钠能够改善悬浮红细胞的变形指数,但对聚集指数和刚性指数无明显影响。Objective To investigate the effects of sodium nitroprussiate on deformability of suspension red blood cells during the storage period.Methods Fifty bags of blood samples（200mL/bag）were collected from blood center.20 mL of blood samples was fractionated to four tubes（5mL/tube）during incubation of blood samples.Three tubes of blood samples has been incubated with different concentrations of sodium nitroprussiate（SNP）at 8-phase（D1,D3,D5,D7,D14,D21,D28,D35）of storage.Concentration of SNP were 1μmol/L（group B）,5μmol/L（group C）and 10μmol/L（group D）.The final tube is control group（group A）without treatment.The relevant indicators of erythrocyte deformability（deformability index,aggregation index,stiffness index）have been detected after incubation.Results Compared to group A,deformability index of groups B and D had no significant changes in storage periods（P〉0.05）,but deformability index of group C were gradually reduced（P〈0.05）,aggregation index and stiffness index had no significant changes in groups B,C and D（P〉0.05）.Conclusion When the concentration of SNP were 5μmol/L,SNP was useful to improve the deformability index of suspension red blood cells,but there were no effects on aggregation index and stiffness index.

关 键 词：硝普钠 悬浮红细胞 红细胞变形性 保存期 

分 类 号：R457.1[医药卫生—治疗学]