壳寡糖及其衍生物抗烟草花叶病毒机理的初步研究  

The Inhibitory Effects of Chitooligosaccharides and its Derivatives on Tobacco Mosaic Virus(TMV)

作　　者：孙翠红[1] 徐翠莲[2] 赵铭钦[1] 王莹[1] 付培培[1] 陈发元[1] 

机构地区：[1]河南农业大学烟草学院,国家烟草栽培生理生化研究基地,郑州450002 [2]河南农业大学理学院,郑州450002

出　　处：《中国烟草科学》2015年第2期87-92,共6页Chinese Tobacco Science

基　　金：河南省烟草公司重点科技攻关项目(HYKJ201015;HYKJ201307)

摘　　要：为了开发一种低毒高效的新型抗烟草花叶病毒抑制剂,利用合成的新型寡糖希夫碱衍生物进行了抗烟草花叶病毒的药效研究。结果表明:(1)在珊西烟上,采用枯斑寄主半叶法进行抗烟草花叶病毒的药剂筛选,壳寡糖及其衍生物都能有效减少叶片感染烟草花叶病毒的枯斑数,以枯斑抑制率为66.39%的水杨醛席夫碱的预防效果最好;(2)在普通烟K326上,壳寡糖及其衍生物可降低侵染病毒烟草中的叶绿素下降幅度,还可以提高叶片中的防御酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和苯丙氨酸解氨酶(PAL)的活性,脯氨酸的含量也得到了积累。In order to develop a kind of the new inhibitor of low toxicity and high efficiency against TMV, the inhibitive effect of the new oligosaccharide Schiff base derivatives against TMV was conducted. The results showed that： （1） on Xanthi-NN, chitosan and its derivatives could all reduce the number of the dry spots infected by TMV effectively by screening potions with the dry spot host half leaf method, and the best result was obtained for salicylaldehyde chitosan Schiff base giving the inhibition rate of 66.39%; （2） on Nicotiana tabacum K326, chitosan and its derivatives can lower the decrease of chlorophyll content, and increase activities of superoxide dismutase （SOD）, peroxidase （POD）, catalase （CAT）, polyphenol oxidase （PPO） and phenylalanine ammonia-lyase （PAL） in inoculated leaves, at the same time the content of proline had also accumulated.

关 键 词：烟草花叶病毒 壳寡糖 壳寡糖席夫碱 防御酶 脯氨酸 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]