Rv0222/ESAT-6融合蛋白对结核病的诊断价值  

Diagnostic Value of Rv0222/ESAT-6Fusion Protein in Tuberculosis

作　　者：张慧涨[1] 范齐文[2] 杨华[3] 黄红梅[1] 方强[1] 

机构地区：[1]复旦大学附属金山医院检验科,上海201508 [2]复旦大学附属公共卫生临床中心检验科,上海201508 [3]上海市肺科医院结核病实验室,上海200433

出　　处：《中国临床医学》2014年第6期636-639,共4页Chinese Journal of Clinical Medicine

基　　金：上海市金山区科学技术创新基金项目(编号:2011-3-01)

摘　　要：目的:探讨Rv0222/ESAT-6融合蛋白对结核病的血清学诊断价值。方法:表达、纯化重组Rv0222/ESAT-6融合蛋白,以其为抗原包板、以酶标抗体为二抗、采用酶联免疫吸附试验(ELISA)检测结核患者、人类免疫缺陷病毒(human immunodeficiency virus,HIV)感染合并结核患者、非结核患者以及正常人血清,评价该融合蛋白对结核病的诊断价值。结果:以结核分枝杆菌感染T淋巴细胞斑点试验(T-SPOT,简称T-SPOT)为金标准,将Rv0222/ESAT-6融合蛋白作为抗原的ELISA诊断结核病的敏感性为86%,特异性为100%;T-SPOT与ELISA对结核病的阳性检出率差异无统计学意义。结论:以Rv0222/ESAT-6融合蛋白作为抗原的ELISA对结核病(包括HIV合并结核病)的血清学诊断有一定价值。Objective：To explore the serodiagnostic value of Rv0222/ESAT-6fusion protein in tuberculosis.Methods：By using purified recombinant Rv0222/ESAT-6 fusion protein as antigen and enzyme-labeled antibody as secondary antibody,the serum of patients with tuberculosis and patients with human immunodeficiency virus（HIV）accompanied by tuberculosis,non-tuberculosis patients and healthy people were detected with enzyme-linked immunosorbent assay（ELISA）.The diagnostic value of the fusion protein in tuberculosis was evaluated.Results：By setting T lymphocyte spot test of infection with Mycobacterium tuberculosis（T-SPOT）as the gold standard,the sensitivity and specificity of ELISA,of which Rv0222/ESAT-6 fusion protein was used as the antigen,in the diagnosis of tuberculosis were 86% and 100%,respectively.There was no statistically significant difference in the positive rate of tuberculosis between T-SPOT and ELISA.Conclusions：The ELISA with Rv0222/ESAT-6 fusion protein as antigen,has certain application value in the serodiagnosis of tuberculosis.

关 键 词：融合蛋白 结核病 Rv0222/ESAT-6融合蛋白 酶联免疫吸附试验 

分 类 号：R446.5[医药卫生—诊断学]