Sn-1,3-specific Interesterification of Soybean Oil with Medium-chain Triacylglycerol Catalyzed by Lipozyme TL IM  

脂肪酶LipozymeTLIM催化合成MLM型结构脂及其1,3专一性的研究(英文)

作　　者：杨宏黎 牟英 陈洪涛 苏春阳 杨天奎 修志龙 

机构地区：[1]School of Life Science and Biotechnology,Dalian University of Technology [2]Wilmar (Shanghai) Biotechnology Research & Development Center Co.,Ltd.

出　　处：《Chinese Journal of Chemical Engineering》2014年第9期1016-1020,共5页中国化学工程学报（英文版）

基　　金：Supported by the National High Technology Research and Development Program of China(2010AA101505);the National Natural Science Foundation of China(21106013)

摘　　要：The structured lipids are produced through sn-l,3-specific interesterification of soybean oil with medium-chain triacylglycerol （MCT） in continuous reactions catalyzed by Thermomyces lanuginose lipase （Lipozyme TL IM）. Cheap Lipozyme TL IM presents similar interesterification degree （ID）, sn-l,3-specificity and residual activity as expensive Rhizomucor miehei in batch reactions. In packed-bed interesterification of soybean oil with Mcr cat- alyzed by Lipozyme TL IM, the residence time has a significant effect on ID, while temperature has a small effect at 45-70 ℃. The sn-l,3-specificity of Lipozyme TI. IM is not satisfactory when reaction temperature is higher than 60 ℃. The optimal residence time and temperature are 30-40 min and 55 ℃, respectively. Among the solvents, including acetone, isopropanol, tert-butanol, and isobutanol, used to recover the activity of the used Lipozyme TL IM, acetone is the most suitable one than the other solvents.The structured lipids are produced through sn-1,3-specific interesterification of soybean oil with medium-chain triacylglycerol(MCT) in continuous reactions catalyzed by Thermomyces lanuginose lipase(Lipozyme TL IM).Cheap Lipozyme TL IM presents similar interesterification degree(ID), sn-1,3-specificity and residual activity as expensive Rhizomucor miehei in batch reactions. In packed-bed interesterification of soybean oil with MCT catalyzed by Lipozyme TL IM, the residence time has a significant effect on ID, while temperature has a small effect at45–70 °C. The sn-1,3-specificity of Lipozyme TL IM is not satisfactory when reaction temperature is higher than60 °C. The optimal residence time and temperature are 30–40 min and 55 °C, respectively. Among the solvents,including acetone, isopropanol, tert-butanol, and isobutanol, used to recover the activity of the used Lipozyme TL IM, acetone is the most suitable one than the other solvents.

关 键 词：Sn- l 3-specificityInteresterificationThermomyces lanuginose lipaseMedium-chain triacylglycerolStructured lipids 

分 类 号：TQ645.6[化学工程—精细化工] TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]