结球甘蓝西园六号种子纯度的SSR和SRAP鉴定  

Seed Purity Identification of Cabbage Variety ‘Xiyuan No.6’ by SSR and SRAP Markers

作　　者：袁天成[1] 司军[1] 宋洪元[1] 任雪松[1] 李成琼[1] 

机构地区：[1]西南大学园艺园林学院/重庆市蔬菜学重点实验室/南方山地园艺学教育部重点实验室,重庆400716

出　　处：《西南大学学报（自然科学版）》2014年第6期41-46,共6页Journal of Southwest University(Natural Science Edition)

基　　金：国家科学支撑计划项目(2012BAD02B01);重庆市农作物良种创新工程项目[CSTC2012GG(80005)];中央高校基本科研业务费资金资助项目(XDJK2010C068)基金资助

摘　　要：用SSR和SRAP标记技术对结球甘蓝西园六号进行品种纯度鉴定,从94对SSR引物和109对SRAP引物中分别筛选出4对条带清晰、多态性较高且具有高度区分度的引物.依据8对引物在西园六号及其亲本的聚丙烯酰胺凝胶电泳检测结果,构建了清晰的品种指纹图谱.通过对特异片段的序列分析,比较了亲本特异片段的碱基差异并揭示了杂交后代中产生非亲条带的本质,将SSR转化为SCAR标记.SSR和SRAP分子标记构成的DNA指纹图谱及SCAR标记,为西园六号品种纯度快速鉴定提供了科学依据.SSR and SRAP markers were used in this study to provide a scientific basis for the rapid identifi- cation of seed purity of cabbage （Brassica oleracea L. var. capitata L. ） ＇ Xiyuan No. 6＇ and its parent ma- terials. Totally, 8 distinct polymorphic primer pairs with high differential rate were selected from 94 SSR primer pairs and 109 SRAP primer pairs, 4 from each. Based on a PAGE （polyacrylamide gel electropho- resis） test of the eight primer pairs, distinct molecular fingerprints of ＇Xiyuan No. 6＇ and its parents were constructed. Further nucleotide sequence analysis of the specific fragments in F1 and its parents revealed the essence of the appearance of non-parental bands in the hybrids.

关 键 词：结球甘蓝 纯度鉴定 非亲条带 序列分析 SCAR 

分 类 号：S635.1[农业科学—蔬菜学]