外源赤霉素对金毛狗孢子萌发和配子体发育的影响  

Effect of GA3 on Spore Germination and Gametophyte Development of Cibotium barometz

作　　者：彭黎立[1,2] 邓洪平[1] 

机构地区：[1]西南大学生命科学学院/三峡库区植物生态与资源重点实验室,重庆400715 [2]四川外国语大学附属外国语学校,重庆40039

出　　处：《西南大学学报（自然科学版）》2014年第8期65-69,共5页Journal of Southwest University(Natural Science Edition)

基　　金：重庆市自然科学基金资助项目(2008BB5256)

摘　　要：为研究外源赤霉素不同处理对金毛狗(Cibotium barometz)孢子萌发和配子体发育的影响,为金毛狗引种繁育、人工栽培及资源保护提供理论依据,采用不同质量浓度外源赤霉素对金毛狗孢子处理不同时间,统计其孢子萌发时间、萌发率、假根数、丝状体形成率、原叶体形成时间、性器官形成时间,分析外源赤霉素对金毛狗孢子萌发和配子体发育的影响.结果发现:0.01mg/L赤霉素处理的金毛狗孢子萌发率、丝状体形成率最高,孢子萌发时间、原叶体形成时间、性器官出现时间最短,不同处理时间之间差异不显著.0.05mg/L赤霉素处理5min假根数最多.低质量浓度赤霉素处理促进金毛狗孢子萌发和配子体发育,高质量浓度赤霉素抑制金毛狗孢子萌发和配子体发育.The spores of Cibotium barometz were incubated on 1/2MS medium and treated with GA3 at 4 concentrations for different time duration, and the time and rate of spore germination, rhizoid number, protonema formation rate, prothallus formation time and sexual organ formation time were recorded so as to study the effects of GA3 on its spore germination and gametophyte development and thus provide useful information for the introduction, multiplication, artificial cultivation and germplasm preservation of the plant. The results showed that of the GA3 concentration treatment designed, 0.01 mg/L gave the highest spore germination rate and protonema formation rate, and the shortest spore germination, prothallus formation and organ formation time. No significant difference was detected among different time duration treatments. The spores had the maximum rhizoid number at 0.05 mg/L GA3 in 5 min .

关 键 词：外源赤霉素 金毛狗 孢子萌发 配子体发育 

分 类 号：Q944[生物学—植物学]