抗稻瘟病基因pi5检测标签的设计及验证  

Design of Detecting Markers to Rice Blast Resistance Gene pi5and Their Validation

作　　者：郑文静[1,2] 丛玲[2] 王妍[3] 赵家铭[1] 张丽霞[1] 陈温福[1] 

机构地区：[1]辽宁省农业科学院,沈阳110161 [2]沈阳农业大学水稻研究所,沈阳110161 [3]沈阳农业大学植物保护学院,沈阳110161

出　　处：《西南大学学报（自然科学版）》2014年第9期15-22,共8页Journal of Southwest University(Natural Science Edition)

基　　金：辽宁省博士启动基金资助项目(20121143);辽宁省科技攻关资助项目"主要农作物生物育种技术研究"(2011208001)

摘　　要：稻瘟病是在世界范围内广泛分布的水稻真菌病害,控制该病的为害必须有效利用抗稻瘟病基因.抗病基因pi5包含2个独立遗传的NBS-LRR类基因Pi5-1和Pi5-2,为了解该基因的抗谱并使其在抗病育种中得以有效利用,本研究利用辽宁地区分离出的6群15个生理小种对携带该基因的单基因系接种,结果表明单基因系对其中12个生理小种表现中抗或抗病,只对其中3个小种表现中感或感病,这说明pi5抗病基因抗谱较宽,是一个可在辽宁地区广为使用的广谱抗稻瘟病基因.为了提高该基因的选择效率,使其在抗病品种选育中发挥更大的作用,本研究根据pi5-1和pi5-2的CDS序列设计特异性引物,并通过基因扩增和测序比对,获得了6对用于育种亲本材料或杂交后代抗稻瘟病基因型检测的标记,为携带pi5抗病基因育种材料的分子标记辅助育种提供了参考.Blast is one of the most devastating rice fungial diseases in the world. ,and efficientl use of the resistance genes is essential Tfor itso control the disease fundamentally ,using resistance genes efficiently is essential .The resistance gene pi5 contains the NBS-LRR （nucleotide binding site-leucine-rich repeats） type genes Pi5-1 and Pi5-2 ,which are of independent inheritance .To ascertain the resistance spectrum of pi5 and promote its utilization efficiency in resistantce breeding ,the single-gene line of pi5 was inoculated with 15 physiological races of rice blast which were separated from Liaoning province and ,which belonged to 6 taxa ,respectively .The results of the inoculation test showed that the single-gene line of pi5 was resistant or moderately resistancte of pi5 to 12 physiological races of the was the middle level and stable resistancepathogen ,and susceptible or moderately susceptible to only 3 physiological races were pathogenic to single gene line of p i5 ,w hich indicated that p i5 is ahas a broad-resistance spectrum resistant gene and is ,therefore ,well-suited to be used in Liaoning province .To promote the utilization efficiency of pi5and applied value in blast resistantce breeding of pi5 ,we designed specific primers according to the CDS （coding sequence） of pi5-1 and pi5-2 ,and by gene amplification and sequence contrasting ,6 pairs of the testing primers were validatedshown to be suitable to be used infor selection of hybrids and parent germplasm as parents ,w hich maybe contribute to promoteing the efficiency and accuracy of resistantce breeding .

关 键 词：稻瘟病 抗病基因 pi5 检测标签 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]