相似穿孔线虫消毒方法筛选及其对线虫繁殖量的影响  

Screening of Surface-Sterilizing Methods for Radopholus similis and Their Influences on Its Reproduction

作　　者：丁莎[1] 徐春玲[1] 谢辉[1] 李静[1] 俞璐[1] 张建峰[1] 袁月[1] 

机构地区：[1]华南农业大学资源环境学院/植物线虫研究室/植物检疫线虫检测与防疫研究中心,广州510642

出　　处：《西南大学学报（自然科学版）》2014年第9期37-43,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家青年科学基金资助项目(31000068);教育部“高等学校博士点学科点专项科研基金”新教师类联合资助项目(20104404120023)

摘　　要：通过比较不同消毒方法对相似穿孔线虫的消毒效果和对其活力的影响,筛选出5种消毒方法,再通过接种胡萝卜愈伤组织繁殖线虫的方法,测定了这些消毒方法对相似穿孔线虫繁殖量的影响.结果表明:供试的5个相似穿孔线虫种群,用硫酸链霉素进行消毒后的雌虫平均繁殖量最大,用次氯酸钠处理后的雌虫平均繁殖量最小,而用氯化汞、洗必泰+氯化汞和硫酸链霉素+氯化汞进行处理后的雌虫平均繁殖量介于上述两者之间,综合消毒效果和对繁殖量的影响2个因素,其中洗必泰+氯化汞是最合适的消毒方法.By comparing the sterilizing effects of different surface-sterilizing methods and their impacts on the vitality of Radopholus similis ,five were chosen to study their influences on the reproduction of R. si-milis cultured on carrot disks .The results showed that of the 5 similar nematode populations used for surface sterilization ,the one sterilized with streptomycin sulfate had the greatest average reproduction amount per female and that sterilized with sodium hypochlorite had the smallest ,and the average reproduction amount per female in the populations sterilized with mercuric chloride ,hibitane plus with mercuric chloride or streptomycin sulfate plus mercuric chloride appeared in between .Thus considering the two factors of sterilizing effects and influences on the reproduction amount ,hibitane plus with mercuric chloride is the most appropriate sterilizing method on R. similis .

关 键 词：相似穿孔线虫 胡萝卜愈伤组织 消毒 繁殖 

分 类 号：S763.16[农业科学—森林保护学]