大花红景天指纹图谱研究  

Study on HPLC Fingerprint of Rhodiola crenulata

作　　者：刘爽[1] 王佩龙[1] 胡益杰 兰小中[2] 廖志华[3] 陈敏[1] 

机构地区：[1]西南大学药学院,发光与实时分析教育部重点实验室,重庆400715 [2]西藏大学农牧学院,西藏林芝860000 [3]西南大学生命科学学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2014年第12期215-221,共7页Journal of Southwest University(Natural Science Edition)

基　　金：国家科技支撑计划子课题资助项目(2011BAI13B06);国家自然科学基金资助项目(81102894)

摘　　要：目的:建立大花红景天药材的HPLC指纹图谱.方法:采用RP-HPLC分析,以WondaSil C18色谱柱(4.6mm×150mm,5μm)为色谱柱,流动相A为0.1%甲酸水(pH=3.2),流动相B为乙腈,梯度洗脱,流速0.8mL/min,柱温25℃,检测波长275nm,进样量10μL.利用中药指纹图谱相似度评价系统(2004A版)对10个批次大花红景天药材HPLC图谱进行分析比较.结果:建立了不同产地大花红景天药材的HPLC指纹图谱,以红景天苷、酪醇为参照峰确立了大花红景天药材指纹图谱中的30个共有峰.结论:该方法具有良好的重现性及稳定性,可用于大花红景天药材的质量管理规范.Objective：To establish the chromatographic fingerprint of the root of Rhodiola crenulata by RP‐HPLC .Methods ：Ten batches of samples from different producing areas were analyzed with a Wonda‐Sil C18 column （4.6 mm × 150mm ,5 μm ） ,gradiently eluted with A （water containing 0.1% methanoic acid ,pH=3.2）‐B （acetonitrile） at 25 ℃ ,and monitored with a UV detector at 275 nm .The flow rate was 0.8 mL/min ,and the sample volume injected was 10 μL .The chromatograms of different batches of R.crenulata were compared by the software of Similarity Evaluation System for Chromatographic Fingerprint of TCM （Version 2004A） .Results ：HPLC Fingerprints of R. crenulata of different origins were estab‐lished with tyrosol and salidroside as the reference compounds .Thirty mutual peaks were selected as the fingerprint peaks of R. crenulata .Conclusion ：The method is simple ,rapid and accurate ,and can be pro‐vided as a scientific and effective way for quality control of R. crenulata .

关 键 词：大花红景天 HPLC指纹图谱 质量控制 酪醇 红景天苷 

分 类 号：R284.1[医药卫生—中药学]