命运分子cNumb在原肠期胚胎的表达及其生物信息学分析  

Expression of determinant Numb in chick embryo at gastrula and its bioinformatics analysis

作　　者：顾取良[1] 陈佳园[2] 王广[3] 徐涛[2] 郐一贺 杨雪松[3] 王丽京[2] 

机构地区：[1]广东药学院基础学院,广东广州510006 [2]广东药学院血管生物学研究所,广东广州510006 [3]暨南大学医学院组织与胚胎学教研室/再生医学教育部重点实验室,广东广州510632

出　　处：《广东药学院学报》2015年第3期383-387,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金项目(31100852)

摘　　要：目的探讨命运分子c Numb在原肠期胚胎中的表达情况及其基因和编码蛋白的基本生物学特征。方法利用原位杂交技术检测c Numb基因在原肠期鸡胚中的表达情况,同时应用生物学软件和在线平台对c Numb基因及其编码蛋白进行生物信息学分析。结果 c Numb mRNA在鸡胚4期开始表达于原条顶端,随发育过程逐渐扩展至亨氏节、头突和神经管,但表达区域仍相对集中。Numb基因在不同物种中具有较高保守性,c Numb编码的蛋白属于胞内蛋白,包含磷酸酪氨酸相互作用结构域和多个潜在磷酸化位点,可参与调节多种细胞生物学过程。结论通过生物信息学分析获得了c Numb基因及其蛋白的生物学特征,为进一步研究Numb在胚胎神经发育中的调控机制奠定了基础。Objective To explore the expression of cell fate determinant Numb homolog of chick（ c Numb） in early embryogenesis and genetic characteristics as well as the encoding protein structure and function information. Methods Numb mRNA expression at gastrula was analyzed by in situ hybridization. The physicochemical properties,cellular location,secondary structural features,motifs / patterns / domains,tertiary structure and post-translational modification events of Numb were predicted by using several bioinformatics tools. Results c Numb mRNA was expressed mainly in the region of primitive streak which develops into nervous system later. A phylogenetic tree has been derived from the consensus analysis of nucleotide sequence of 16 species,which indicated that Numb is highly conserved. Moreover,preliminary sequence analysis showed that c Numb is a putative intracellular protein and has a phosphotyrosine binding domain and multiple phosphorylation sites. Conclusion The expression pattern of c Numb in early chick embryo and its bioinformatics analysis provide an evidence for further study of Numb in embryonic neurodevelopment.

关 键 词：NUMB 胚胎发育 生物信息学分析 

分 类 号：Q811.4[生物学—生物工程]