EFFECTS OF MODERATE NOISE EXPOSURE ON HEARING FUNCTION IN C57BL/6J MICE  

作　　者：SHI Chuang SHI Lei JIANG Xuejun YANG Shiming LIU Ke 

机构地区：[1]Department of Otolaryngology Head and Neck Surgery,The First Affiliated Hospital of China Medical University [2]Department of Otolaryngology Head and Neck surgery,Shiyan Taihe Hospital [3]Department of Otolaryngology Head and Neck surgery,Institute of Otolaryngology,Chinese PLA General Hospital

出　　处：《Journal of Otology》2014年第2期81-85,共5页中华耳科学杂志（英文版）

基　　金：supported by grants from the Liaoning Science and Technology Project(No.2011225017,2012225021);the National Basic Research Program of China(973 Program)(2012CB967900,2012CB967901);supported by the grants from the Beijing Natural Science Foundation(5122040);the China Postdoctoral Science Foundation(201003779,20100470103);the National Natural Science Foundation of China(NSFC,31040038)

摘　　要：Objective To study characteristics of hearing loss after exposure to moderate noise exposure in C57BL/6J mice. Methods Male C57BL/6J mice with normal hearing at age of 5-6 weeks were chosen for this study. The mice were randomly sclccted to be studied immediately after exposure （Group P0）, or 1 day （Group P1）, 3 days （Group P3）, 7 day （Group P7） or 14 days （P14） after exposure. Their before exposure condition served as the normal control. All mice were exposed to a broad-band white noise at 100 dB SPL for 2 hours, ABR thresholds were used to estimate hearing status at each time point. Results ABR threshold elevation was seen at every tested frequency at P0 （P〈0.01）. Elevation at high-frequencies （16 kHz and 32 kHz） was greater than at lower frequencies （4 kHz and 8 kHz, P〈0.05）. From P1 to P14, ABR thresholds continuously improved, and there was no significant difference between P14 and before exposure （P〉0.05）. Conclusion There is a frequency specific re- sponse to 100 dB SPL broad-band white noise in C57BL/6J mice, with the high-frequency being more susceptible. Hearing loss induced by moderate noise exposure appears reversible in C57BL/6J mice.Objective To study characteristics of hearing loss after exposure to moderate noise exposure in C57BL/6J mice. Methods Male C57BL/6J mice with normal hearing at age of 5-6 weeks were chosen for this study. The mice were randomly sclccted to be studied immediately after exposure （Group P0）, or 1 day （Group P1）, 3 days （Group P3）, 7 day （Group P7） or 14 days （P14） after exposure. Their before exposure condition served as the normal control. All mice were exposed to a broad-band white noise at 100 dB SPL for 2 hours, ABR thresholds were used to estimate hearing status at each time point. Results ABR threshold elevation was seen at every tested frequency at P0 （P〈0.01）. Elevation at high-frequencies （16 kHz and 32 kHz） was greater than at lower frequencies （4 kHz and 8 kHz, P〈0.05）. From P1 to P14, ABR thresholds continuously improved, and there was no significant difference between P14 and before exposure （P〉0.05）. Conclusion There is a frequency specific re- sponse to 100 dB SPL broad-band white noise in C57BL/6J mice, with the high-frequency being more susceptible. Hearing loss induced by moderate noise exposure appears reversible in C57BL/6J mice.

关 键 词：C57BL/6J mice Noise exposure Hearing loss Temporary noise-induced hearing loss ABR threshold shift 

分 类 号：R764[医药卫生—耳鼻咽喉科]