好视力眼贴对小鼠耳廓血流量及皮肤超氧化物歧化酶的影响  

Effects of HAO SHI LI YAN TIE on the blood flow volume of auricle plane and skin SOD activity in mice

作　　者：张硕峰[1] 张秋霞 武汀[1] 吴金英[1] 贾占红[1] 孙建宁[1] 

机构地区：[1]北京中医药大学中药药理系,北京100102 [2]北京好视力眼科医学研发中心

出　　处：《中华眼科医学杂志（电子版）》2013年第6期7-9,共3页Chinese Journal of Ophthalmologic Medicine(Electronic Edition)

摘　　要：目的观察好视力眼贴对小鼠耳廓血流量的影响和清除自由基的作用。方法将麻醉小鼠俯位固定,并固定右侧耳廓,使用激光多普勒血流仪,测定右侧耳廓远心端组织血流量,待血流平稳后,在右侧耳廓近心端,分别滴加8%、4%、2%浓度的药液0.1ml/只,并记录加药前、加药后5、10、15、20、25min的血流值;采用8%、4%、2%浓度的药液浸泡小鼠尾部的给药方法,给药5d后取小鼠尾部皮肤,匀浆,测定小鼠尾部皮肤SOD活性。实验结果采用SSPS 17.0统计学软件对数据进行统计分析,组间的两两比较采用LSD-t检验。结果对照组小鼠耳廓部血流量减少8.67%-18.02%,好视力眼贴可抑制小鼠耳廓部血流量的减少,其中8%浓度作用明显,给药后5-10min时血流量仅减少（1.78±25.28）%,与对照组相比较差异有统计学意义（P〈0.05）。4%、2%外用分别可使小鼠尾部皮肤SOD活性增强101.3%,61.6%（对照组12.56±3.27,4%组为25.29±16.71,2%组为20.30±11.91,与对照组相比较P〈0.05）。结论好视力眼贴可增加用药局部血流量,增强用药局部清除自由基能力。Objective To observe the effects of the blood flow volume of mice auricle plane and radical scavenging activity of Hao Shi Li Yan Tie（HSLYT）.Methods Mice anesthetized with 10%chloral hydrate（350mg/kg,i.p.）were fixed onto the platform in a prone position,and the blood flow volume of mice auricle plane was recorded with the laser doppler flood-flow monitor before administration and 5,10,15,20,25 minutes after administration of 8%,4%,2% solution of HSLYT respectively.The sampling time for each time point was 5minutes.The SOD activity of tail skin of mice which were soaked in 8%,4%,2% solution of HSLYT Tie for 5days was measured.Results The blood flow volume of mice auricle plane of control dropped 8.67%-18.02%.HSLYT can restrain the decrease of blood flow volume of mice auricle plane.The effect of 8% solution of HSLYT after administration 5minutes obviously decreased 1.78% ±25.28%（P〈0.05）.The SOD activity of tail skin of mice was increased by 4% and 2%solution of HSLYT（control group 12.56±3.27,4% group25.29±16.71,2% group 20.30±11.91,P0.05）.Conclusion Local administration of HSLYT could increase blood flow volume and SOD activity.

关 键 词：视疲劳 超氧化物歧化酶 血流量 

分 类 号：R285.5[医药卫生—中药学]