新疆两色金鸡菊总黄酮含量测定显色反应体系  

The determination method of total flavonoids in Coreopsis tinctoria Nutt. from Xinjiang in different chromogenic reaction system by spectrophotometry

作　　者：姚新成[1,2] 王新兵[1,2] 张婷[1] 陈文[1,2] 唐辉[1,2] 

机构地区：[1]石河子大学药学院,新疆石河子市832000 [2]新疆特种植物药资源教育部重点实验室,新疆石河子市832000

出　　处：《暨南大学学报（自然科学与医学版）》2015年第3期222-227,共6页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家“重大新药创制”科技重大专项(2011ZX09401-007);新疆生产兵团重点领域科技攻关项目(2014BA031);石河子大学高层次人才科研启动项目(RCZX201328)

摘　　要：目的:为准确测定新疆两色金鸡菊(Coreopsis tinctoria Nutt.)中总黄酮的含量,选择合适的显色反应体系.方法:通过比较Na NO2-Al(NO3)3/Al Cl3-Na OH和Al(NO3)3/Al Cl3+KAc两种反应显色体系条件下两色金鸡菊提取物和对照品芦丁、槲皮素和槲皮苷的连续波长扫描图谱,考察两种反应体系光谱吸收曲线和最大吸收峰值!max,进而选择适合测定两色金鸡菊中总黄酮的反应体系.结果:样品与槲皮素或槲皮苷对照品在Al(NO3)3/Al Cl3+KAc显色反应体系下产生的光谱吸收曲线相似,最大吸收峰峰位值更接近.因此,可以用该显色反应体系快速测定样品中总黄酮的含量.结论:该测定方法简单易行,能准确测定新疆两色金鸡菊中总黄酮的含量.Aim：To select a chromogenic reaction system for accurate determination of total flavonoids in Coreopsis tinctoria Nutt.from Xinjiang.Methods：The spectrum of C.tinctoria flowering tops （CTF） extract,rutin,quercetin,quercitrin standards were compared in NaNO2-Al（NO3 ）3 /AlCl3-NaOH and Al （NO3 ）3 /AlCl3 ＋KAc reaction system,respectively.The absorption curve and the maximum absorption peak value were investigated for selecting a suitable reaction system to determine the content of total fla-vonoids in C.tinctoria.Results：The spectrum of CTF extract,quercetin and quercitrin standard were similar in Al（NO3 ）3 /AlCl3 ＋KAc reaction system,and the maximum absorption peak values were also closer to each other.Therefore,the Al（NO3 ）3 /AlCl3 ＋KAc reaction system could be used to determine the content of total flavonoids in C.tinctoria.Conclusion：The method was simple,accurate and could be used to determine the content of total flavonoids in C.tinctoria Nutt.

关 键 词：两色金鸡菊 总黄酮 显色反应体系 分光光度法 

分 类 号：O657.32[理学—分析化学]