参麦注射液中有效成分的含量测定  

The Determination of the content of active ingredients in Shenmai Injection

作　　者：苏家辉[1] 林炳国[1] 吴舜芳[1] 蓝义琨[1] 

机构地区：[1]佛山市中医院,广东佛山528000

出　　处：《中医临床研究》2015年第15期140-142,共3页Clinical Journal Of Chinese Medicine

摘　　要：目的：测定参麦注射液中主要有效成分人参皂苷Rg1、人参皂苷Re、人参皂苷Rf和人参皂苷Rb1的含量。方法：采用高效液相色谱法进行梯度洗脱,色谱柱为BDS Hypersil C18色谱柱（250 mm×4.6 mm,5.0μm）,流动相为乙腈：水=25：75（0~40 min）,35：65（40~70 min）,检测波长为210 nm,进样量为15μL,流速为1.0 m L/min,柱温为35℃。结果：人参皂苷Rg1在0.3985~3.985μg（r=0.9998）、人参皂苷Re在0.4104~4.104μg（r=0.9997）、人参皂苷Rf在0.2998~2.998μg（r=0.9998）、人参皂苷Rb1在0.6042~6.042μg（r=0.9998）范围内具有稳定的线性关系;平均回收率分别为96.97%、98.47%、96.62%、98.55%（n=6）,RSD值分别为1.36%、1.22%、1.54%、1.67%。结论：高效液相色谱法用于参麦注射液有效成分的含量测定中,方法简单可靠,重复性好,含量测定准确,灵敏度高,可以作为参麦注射液含量测定和质量控制的方法之一。Objective： To determine the content of the main active ingredients of ginsenosides Rg1, ginsenoside Re, ginsenoside Rf and ginsenoside Rb1 in Shenmai Injection. Methods： The high performance liquid chromatography was taken for gradient, elution column was BDS Hypersil C18 column（250 mm × 4.6 mm, 5.0 μm）, the mobile phase of acetonitrile was water： 25： 75（0 ~40 min）, 35： 65（ 40~70 min）, the detection wavelength was 210 nm, the injection volume was 15 μL, the flow rate was 1.0 m L / min, and the column temperature was 35 ℃. Results： Ginsenosides Rg1 had stable linear relationship in 0.3985 ~ 3.985μg（r = 0.9998）, ginsenoside Re in 0.4104~4.104 μg（r = 0.9997）, ginsenoside Rf in 0.2998~2.998μg（r = 0.9998）, ginsenoside Rb1 at 0.6042~6.042 μg（r = 0.9998）, and the average recovery rates were 96.97%, 98.47%, 96.62%, 98.55%（n = 6）, RSD values were 1.36%, 1.22%, 1.54%, 1.67 %. Conclusion： HPLC in the application of the determination of the active ingredient in Shenmai injection, the method is simple, reliable, reproducible and accurate. It is of high sensitivity, which can be used as one of the methods for the determination and quality control for Shenmai Injection.

关 键 词：参麦注射液 高效液相色谱法 人参皂苷RG1 人参皂苷RE 人参皂苷Rf 人参皂苷RB1 

分 类 号：R283[医药卫生—中药学]