SSR标记变性聚丙烯酰胺凝胶快速银染方法的建立  

Establishment of Rapid Silver-stained Method of Denaturing Polyacrylamide Gel of Microsatellite Markers

作　　者：李建武[1] 李宁[2] 

机构地区：[1]甘肃省农业科学院马铃薯研究所,甘肃兰州730070 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《中国马铃薯》2015年第3期136-140,共5页Chinese Potato Journal

基　　金：国家自然基金"四倍体马铃薯分子遗传图谱的构建与淀粉含量等重要农艺性状的QTL定位研究(31160299)";甘肃省省青年科技基金计划"甘肃省马铃薯主栽品种指纹图谱的构建及遗传多样性分析(1308RJYA047)";甘肃省农业科学院农业科技创新专项"甘肃省马铃薯主栽品种及重要种质资源DNA指纹图谱的构建(2013GAAS43)"

摘　　要：针对传统聚丙烯酰胺凝胶电泳银染方法存在染色时间长、步骤繁琐,难以大批量试验等诸多不利因素,对改良的银染检测方法与文献报道的4种银染检测方法的染色效果、时间及试剂成本等进行对比分析。结果表明,改良方法省略前期的漂洗与固定步骤,只进行银染、漂洗、显影和终止4个步骤,整个银染过程耗时12 min,较其他方法耗时少,目标片段较清晰,试剂用量少,过程较为简单,可批量操作。Traditional silver staining method of denaturing polyacrylamide gel electrophoresis is time- consuming,tedious, and difficult for large- scale operation. An improved silver staining method and four other reported methods were compared in terms of staining quality, time consumption, and reagent cost. For the improved silver staining methods, rinsing and fixing were omitted at the early stage, and only silver staining, rinsing, developing and ending were needed, taking only 12 min totally in the process. This improved method was time efficient, and showed clear target fragments, which used less amount of reagents and was easy to operate when compared with other reported methods.

关 键 词：SSR标记 变性聚丙烯酰胺凝胶 银染 

分 类 号：Q94-33[生物学—植物学]