Studies on the Isolation, Identification and In Vitro Growth Rates of the Three Pathogenic Fungi from Panax notoginseng Cultivated in Wenshan Eparchy  

文山三七三种病原真菌的分离、鉴定及其体外生长速率的研究（英文）

作　　者：王文亚[1] 赵昶灵[1,2] 陈中坚[3,4] 文国松[1,2] 魏富刚 龙廷菊 李孙文[5] 王崇德[6] 

机构地区：[1]云南农业大学农学与生物技术学院,云南昆明650201 [2]云南农业大学特色资源植物改良与利用研究所,云南昆明650201 [3]文山市苗乡三七实业有限公司,云南文山663000 [4]文山学院三七研究院,云南文山663000 [5]云南农业大学农科专业基础实验教学中心,云南昆明650201 [6]云南农业大学植物保护学院,云南昆明650201

出　　处：《Agricultural Science & Technology》2015年第6期1165-1171,1258,共8页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31060045,31260091,31460065)~~

摘　　要：Objective] The aim of this study was to simultaneously isolate and identify the main pathogenic fungi of the root rot, black spot and round spot from the Panax notoginseng plants cultivated in Wenshan Eparchy of Yunnan Province of China. [Method] The pathogenic fungi were isolated and purified by using potato dextrose agar （PDA） medium. The morphological identification was accomplished first according to the colony forms of the fungi when cultivated in vitro, then accord-ing to the symptom characteristics and colony forms of the re-isolated fungi in the reverse inoculation experiments. The molecular identification was performed accord-ing to the amplification and alignment of the internal transcribed space （ITS） se-quences of the fungi. The increases of the diameters and thickness of the colonies of the fungi cultivated in vitro were employed to indicate the growth rates of the fungi. [Results] The consistency of the colony forms and symptom characteristics and the 96%-99% similarities revealed in the ITS sequence alignments al proved that the main pathogenic fungi of the root rot, black spot and round spot of the P. notoginseng plants raised in Wenshan were Cylindrocarpon didymium, Alternaria panax and Mycocentrospora acerina, respectively. When cultivated in vitro in the same temperature, humidity and il umination, the increases of the colony diameters and thickness of C. didymium were the highest, fol owed by those of A. panax, then those of M. acerina. During different cultivation periods, the differences of the colony diameters and thickness of the three fungi al reached extremely significant level. However, at the same cultivation time, the differences of the diameters and thickness among the three fungi only reached significant level. [Conclusion] The main pathogenic fungi which result in the root rot, black spot and round spot of the P. notoginseng in Wenshan are C. didymium, A. panax and M. acerina, respec-tively. When these three diseases break out at the same time, the root rot wil spread faste[目的]分离、鉴定文山三七根腐病、黑斑病和圆斑病的主要病原真菌,并探明其体外生长速率。[方法]用马铃薯葡聚糖琼脂培养基分离、纯化病原真菌,以真菌体外培养时的菌落形态和回接试验中的病兆特征和再分离真菌的菌落形态进行形态鉴定,以真菌ITS序列扩增与比对进行分子鉴定,用体外培养时病原真菌菌落直径和厚度的增加速率来表征其生长速率。[结果]文山三七根腐病、黑斑病和圆斑病的主要病原真菌分别为双孢柱孢、人参链格孢和槭菌刺孢。体外培养时,双孢柱孢菌落的直径和厚度增大最快,其次为人参链格孢的,槭菌刺孢的则最慢;在同一培养时间,不同真菌的菌落直径、厚度间的各自差异仅达到显著水平。[结论]文山三七根腐病、黑斑病和圆斑病的主要病原真菌分别为双孢柱孢、人参链格孢和槭菌刺孢,当三种病害同时发生时,根腐病将蔓延最快,黑斑病次之,圆斑病则最慢。

关 键 词：Panax notoginseng cultivated in Wenshan Eparchy Root rot black spot and round spot Pathogenic fungus Growth rate in vitro 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]