Effects of Glycidamide on Activity and Progesterone Biosynthesis of Rat R2C Leydig Cells  

环氧丙烯酰胺对睾丸间质细胞R2C活性及孕酮合成功能的影响（英文）

作　　者：许伟[1] 孙建霞[2] 白顺[1] 李名薇 文罗娜 刘洁生[1] 李宏业[1] 黄亚东[1] 白卫滨[1] 

机构地区：[1]暨南大学生命科学学院食品科学与工程系,广东广州510632 [2]广东工业大学轻工化工学院,广东广州510006

出　　处：《Agricultural Science & Technology》2015年第6期1187-1190,共4页农业科学与技术（英文版）

基　　金：Supported by Training Program for Outstanding Young Teachers in Higher Education Institutions,Guangdong Province(Yq2013024);Program for New Century Excellent Talents in University of Ministry of Education of China;National Natural Science Foundation of China(31201402,31201340)~~

摘　　要：Objective] This study aimed to investigate the effects of glycidamide （GA） on growth and progesterone biosynthesis of rat R2C Leydig cel s cultured in vitro. [Method] The R2C Leydig cel s were treated with GA with concentrations of 0.25, 0.5, 0.75, 1, 2, 4 and 6 mmol/L respectively for 48 h. The IC25, IC50 and IC75 values of GA were al detected by MTT assay. The Leydig cel s were treated with GA at concentrations of IC25, IC50 and IC75 respectively for 48 h, and then the morphology of Leydig cel s was observed. After the Leydig cel s were treated with GA for 4 h, the cel ular DNA damage was measured by the comet assay technique; and after the Leydig cel s were with treated with GA for 24 h, the progesterone biosynthesis amount was detected by radioimmunoassay （RIA）. [Result] GA could inhibit the via-bility of R2C Leydig cel s, and its IC25, IC50 and IC75 were 0.635, 0.872 and 1.198 mmol/L, respectively. The GA at concentrations of IC25, IC50 and IC75 affected the growth and morphology of rat R2C Leydig cel s in varying degrees. The 4-h treat-ment of GA could significantly damage the DNA of R2C Leydig cel s, and the 24-h treatment of GA at concentrations of IC25, IC50 and IC75 al reduced the progesterone biosynthesis amount. [Conclusion] GA could inhibit the growth and progesterone biosynthesis of rat R2C Leydig cel s.[目的]研究环氧丙烯酰胺(GA)对体外培养的R2C细胞生长及孕酮合成的影响。[方法]用0.25、0.5、0.75、1、2、4、6 mmol/L浓度的GA作用于细胞48 h,MTT法获得GA作用浓度的IC25,IC50及IC75。用以上3种浓度的GA刺激细胞48 h,观察细胞形态。GA作用于细胞4 h后,通过彗星试验(Comet Assay)检测细胞DNA的损伤程度;放射免疫法(RIA)测定GA刺激细胞24 h后的孕酮合成量。[结果]GA能抑制R2C细胞活性,其IC25、IC50、IC75分别为0.635、0.872及1.198 mmol/L;3种浓度的GA能不同程度的影响细胞生长形态;作用4 h对R2C细胞DNA有损伤作用;各浓度组作用24 h后均能降低孕酮的合成量。[结论]GA能抑制R2C细胞增长及孕酮合成能力。

关 键 词：GLYCIDAMIDE R2C Leydig cel s Cel activity DNA damage Proges-terone 

分 类 号：S859.8[农业科学—临床兽医学]