垂盆草水煎液对不同小鼠急性肝损伤模型的疗效作用  

Effect of Sedum Sarmentosum on acute liver injury-induced by different causes in mice

作　　者：刘三海 李蕊[2,3] 乔艳雪 陈芯[1] 张楠楠[2,3] 张剑平[2,3] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]首都医科大学附属北京地坛医院传染病研究所,北京100015 [3]新发突发传染病研究北京市重点实验室,北京100015

出　　处：《中国肝脏病杂志（电子版）》2015年第1期37-42,共6页Chinese Journal of Liver Diseases：Electronic Version

基　　金：国家自然科学基金(No:81273743)

摘　　要：目的复制四氯化碳（CCl4）、D-氨基半乳糖胺联合内毒素（D-Gal N/LPS）、D-氨基半乳糖胺联合肿瘤坏死因子-α（D-Gal N/TNF-α）及刀豆蛋白A（Con A）诱导小鼠急性肝损伤模型,筛选针对垂盆草（Sedum Sarmentosum,SS）水煎液有效的急性肝损伤小鼠模型。方法采用152只雄性6~8周龄C57BL/6小鼠,其中80只小鼠随机分为CCl4＋H2O组、CCl4＋SS组、D-Gal N/LPS＋H2O组、D-Gal N/LPS＋SS组、D-Gal N/TNF-α＋H2O组、D-Gal N/TNF-α＋SS组、Con A＋H2O组及Con A＋SS组共8组,每组10只,观察各组生存率。其余72只小鼠随机分为Normal组、CCl4＋H2O肝功能组、CCl4＋SS肝功能组、D-Gal N/LPS＋H2O肝功能组、D-Gal N/LPS＋SS肝功能组、D-Gal N/TNF-α＋H2O肝功能组、D-Gal N/TNF-α＋SS肝功能组、Con A＋H2O肝功能组及Con A＋SS肝功能组共9组,每组8只,检测血浆ALT、AST和乳酸脱氢酶（LDH）水平。结果垂盆草水煎液将CCl4小鼠急性肝损伤模型的生存率由55%提高到90%（P=0.0128）。造模24小时后,CCl4＋SS组ALT为（5500.00±426.20）U/L,AST为（4383.00±358.00）U/L,LDH为（6764.00±691.30）U/L,分别低于CCl4＋H2O组ALT[（10521.00±1374.00）U/L]、AST[（7328.00±947.80）U/L]、LDH[（13589.00±1542.00）U/L],差异均有统计学意义（P均〈0.05）。结论垂盆草水煎液对CCl4诱导小鼠急性肝损伤模型有保护作用。Objective To study an appropriate murine model for this research, the mice were administrated by gastrogavage with CCl4, D-Gal N/LPS, D-Gal N/TNF-α and Con A to induce acute liver injury in different group, respectively. Methods The 80 mice were randomly separated from 152 male mice（6-8 weeks）. The 80 mice were randomly divided into CCl4 ＋ H2 O group, CCl4 ＋ Sedum Sarmentosum（SS） group, D-Gal N/LPS ＋ H2 O group, D-Gal N/LPS ＋ SS group, D-Gal N/TNF-α ＋ H2 O group, D-Gal N/TNF-α ＋ SS group, Con A ＋ H2 O group, and Con A ＋ SS group（10 mice per group） with the injection of CCl4, D-Gal N/LPS, D-Gal N/TNF-α and Con A in different groups, and all the mice were administraged by gastrogavage with SS at same time. The survival rate were observed. The 72 mice were randomly divided into Normal group, CCl4＋ H2 O group, CCl4 ＋ Sedum Sarmentosum（SS） group, D-Gal N/LPS ＋ H2 O group, D-Gal N/LPS ＋ SS group, D-Gal N/TNF-α ＋ H2 O group, D-Gal N/TNF-α ＋ SS group, Con A ＋ H2 O group, and Con A ＋ SS group（8 mice per group）. The mice were sacrificed and plasma alanine aminotransferase（ALT）, aspartate aminotransferase（AST） and lactate dehydrogenase（LDH） were measured. Results The survival rate of CCl4＋ SS group mice was elevated from 55% to 90%（P = 0.0128）. After 24 hours challenge, the serum ALT level was（5500.00 ± 426.20） U/L, AST（4383.00 ± 358.00） U/L and LDH（6764.00 ± 691.30） U/L of CCl4 ＋SS group, lower than CCl4 ＋ H2 O group ALT（10521.00 ± 1374.00） U/L, AST（7328.00 ± 947.80） U/L and LDH（13589.00 ± 1542.00） U/L（P〈0.05）. Conclusions Sedum Sarmentosum has an obvious protecting effect for acute liver injury induced by CCl4 in mice.

关 键 词：垂盆草 急性肝损伤 C57BL/6小鼠 

分 类 号：R285.5[医药卫生—中药学]