绞股蓝皂苷联合银杏叶提取物对2型糖尿病合并NAFLD大鼠脂肪肝程度的影响  

Effect of the degrees of fatty liver disease in rats with type 2 diabetes mellitus and nonalcoholic fatty liver disease was treated by gypenosides and ginkgo biloba extract mixture

作　　者：张霞[1,2] 雷飞飞[1] 李芳[1] 谭华炳[1] 

机构地区：[1]湖北医药学院附属人民医院肝病研究室,湖北十堰市442000 [2]湖北房县人民医院内分泌科,湖北房县442100

出　　处：《中国肝脏病杂志（电子版）》2015年第1期55-58,共4页Chinese Journal of Liver Diseases：Electronic Version

基　　金：2009年十堰市科技局科技攻关指导项目(2009zd009)

摘　　要：目的观察绞股蓝皂苷(GPS)和银杏叶提取物(GBE)混合物对2型糖尿病(T2DM)合并非酒精性脂肪性肝病(NAFLD)大鼠脂肪肝程度的影响。方法 40只SPF级雄性SD大鼠随机分为空白组、T2DM合并NAFLD模型组,造模周期8周。将T2DM合并NAFLD模型随机分为模型组(给予同等体积的纯净水灌胃)、对照组[给予GPS 0.5 g/(kg·d)灌胃]、治疗组[给予GPS 0.5 g/(kg·d)加GBE0.1 g/(kg·d)灌胃]。继续予高糖高脂饲料,自由饮水;治疗周期为6周;实验周期14周。检测血糖(BS)、甘油三酯(TG)、总胆固醇(TC)、ALT、AST。检测肝组织TG及肝脏病理学。结果空白组、模型组、治疗组、对照组BS水平分别为(5.50±0.51)mmol/L、(18.76±4.20)mmol/L、(10.78±3.38)mmol/L、(14.62±3.32)mmol/L,治疗组、对照组低于模型组(P<0.01),治疗组低于对照组(P<0.01)。空白组、模型组、治疗组、对照组TG水平分别为(0.81±0.11)mmol/L、(1.29±0.14)mmol/L、(0.90±0.09)mmol/L、(1.09±0.10)mmol/L,治疗组、对照组低于模型组(P<0.01),治疗组低于对照组(P<0.05)。空白组、模型组、治疗组、对照组TC水平分别为(1.39±0.13)mmol/L、(3.69±0.27)mmol/L、(2.59±0.19)mmol/L、(3.11±0.18)mmol/L,治疗组、对照组低于模型组(P均<0.01),治疗组低于对照组(P<0.01)。空白组、模型组、治疗组、对照组ALT水平分别为(35.12±3.34)U/L、(60.68±5.12)U/L、(42.13±4.99)U/L、(50.12±4.68)U/L,治疗组、对照组低于模型组(P均<0.01),治疗组低于对照组(P<0.05)。空白组、模型组、治疗组、对照组AST水平分别为(81.15±7.14)U/L、(168.12±10.49)U/L、(112.23±10.12)U/L、(139.12±11.68)U/L,治疗组、对照组低于模型组(P<0.01),治疗组低于对照组(P<0.01)。空白组、模型组、治疗组、对照组肝组织TG水平分别为(30.26±2.48)mg/G、(228.46±8.48)mg/G、(153.12±9.98)mg/G、(196.24±9.78)mg/G,治疗组、对照组低于模型组(P<0.05),治疗组低于对照组(P<0.01)。空白组为正常肝组织,模型组为重度�Objective To observe effect of the degrees of fatty liver disease in rat with type 2 diabetes mellitus and nonalcoholic fatty liver disease was inhibited by Gypenosides and Ginkgo Biloba Extract Mixture. Methods Total of 40 SPF male SD rats, body mass 220-250 g, were randomly divided into blank control group, model group with type two diabetes mellitus and nonalcoholic fatty liver disease. After eight weeks the NAFLD model in rats was maked, model group were divided into three groups： treatment group were perfused with 500 mg/（kg·d） GPS and 0.1g/（kg·d） ginkgo biloba extract mixture, control group were perfused with 500 mg/（kg·d） GPS, model group were perfused with the same volume of water, and high fat diet at the same time, the experimental period was six weeks. The experimental period was 14 weeks. Blood sugar, triglycerides, total cholesterol（TC）, ALT, AST in the plasma in rat was test. It was tested that triglycerides in the liver tissue. The Liver tissue was detected by pathology. Results BS levels in plasma： blank control group（5.50 ± 0.51） mmol/L, model group（18.76 ± 4.20） mmol/L, treatment group（10.78 ± 3.389） mmol/L, control group（14.62 ± 3.32） mmol/L, treatment group and control group was lower than model group, there was a significant difference（P〈0.01）, treatment group was lower than control group, there was a significant difference（P〈0.05）. TG levels in plasma： blank control group（0.81 ± 0.11） mmol/L, model group（1.29 ± 0.14） mmol/L, treatment group（0.90 ± 0.09） mmol/L, control group（1.09 ± 0.10） mmol/L, treatment group and control group was lower than model group, there was a significant difference（P〈0.01）, treatment group was lower than and control group, there was a significant difference（P〈0.05）. TC levels in plasma： blank control group（1.39 ± 0.13） mmol/L, model group（3.69 ± 0.27） mmol/L, treatment group（2.59 ± 0.19） mmol/L, control group（3.11 ± 0.18） mmol/L, treatment group and control

关 键 词：2型糖尿病合并非酒精性脂肪性肝病 绞股蓝提取物和银杏叶提取物混合物 脂肪肝程度 影响 动物 

分 类 号：R285.5[医药卫生—中药学]