野生土坛树种群遗传多样性的SRAP分析  

Population genetic diversity of wild Alangium salviifolium detected by SRAP marks

作　　者：陈杰[1,2] 韩维栋[2] 莫定鸣 王秀丽[4] 

机构地区：[1]湛江市森林公园管理处,广东湛江524012 [2]广东海洋大学农学院,广东湛江524088 [3]湛江市农业科学研究院,广东湛江524094 [4]厦门大学环境与生态学院,广东厦门361102

出　　处：《广东农业科学》2015年第10期127-132,共6页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31170511)

摘　　要：采用SRAP分子标记技术,对我国野生土坛树种群的遗传多样性进行分析。40对引物对8个土坛树种群共47个个体的样品DNA进行扩增。结果表明:物种水平上,多态位点百分率PPB为40.40%,Nei’s基因多样性指数H为0.4027,Shannon’s信息指数I为0.5874;在种群水平上,多态位点百分率平均为32.77%,Nei’s基因多样性指数平均为0.2992,Shannon’s信息指数平均为0.4373;种群内遗传多样性Hs为0.2992,种群总的遗传多样性Ht为0.4027。基因分化系数Gst为0.2570,种群间的遗传分化程度较低;种群间基因流Nm为1.4455。种群间的平均距离为0.2118。利用UPGMA法聚类可将8个群体划分为2大类,高桥独立为一群,其他为一大群;证明亲缘关系与地理距离存在一定的相关性。47个材料按UPGMA法聚类分析,结果不与同一种群的个体聚在一起。初步推测土坛树由三墩和琼山一带向四周扩散而来。The SRAP markers technology was used to analyze the genetic diversity of wild Alangium salviifolium populations in this paper.DNA samples of 47 individuals in eight A.salviifolium populations were amplied by 40 primers.Analysis showed that PPB（percentage of polymorphic loci） was 40.40%,H（Nei＇s genetic diversity） was 0.4027,I（Shannon＇s information index） was 0.5874 at the species level;PPB was 32.77%,H was 0.2992,I was 0.4373 at the population level;Hs was 0.2992,Ht was 0.4027;Gst（Genetic differentiation coefficient） was 0.2570,indicating that the degree of genetic differentiation was low.The gene flow among the populations was 1.4455 and the mean pair-wise genetic distance was 0.2118.The eight groups could be divided into two broad categories by UPGMA method.Gaoqiao group was independence category,and others joined as one category.It proved that there was certainly relationship between their kinships and geographical distances.According to UPGMA clustering analysis,47 materials did not join together in the same category with individuals of their own population.Preliminary results indicated that A.salviifolium spreaded from Sandun and Qiongshan to the surrounding areas.

关 键 词：土坛树 种群 遗传多样性 SRAP 

分 类 号：Q346.5[生物学—遗传学]