亚硫酸氢钠-过氧化氢超微弱化学发光体系用于核黄素检测  

Detection of Riboflavin Based on Sodium Bisulfite-Hydrogen Peroxide Ultra-weak Chemiluminescence System

作　　者：林珍[1] 朱连杰[1] 王菲菲[1] 吴娜梅 林新华[1] 

机构地区：[1]福建医科大学药学院药物分析系,福建福州350108

出　　处：《分析测试学报》2015年第5期595-599,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(21305015);国家"863"计划项目(2012AA022604);福建省自然科学基金项目(2014J05014);福建医科大学博士启动基金(2012bs003)

摘　　要：亚硫酸氢钠（Na HSO3）和过氧化氢（H2O2）反应可产生微弱的化学发光。核黄素对亚硫酸氢钠和过氧化氢的化学发光有极大的增强作用,且化学发光强度在0.02~2.0μg/m L范围内与核黄素浓度呈良好的线性关系。据此,建立了核黄素的高灵敏化学发光分析方法,方法检出限为0.007μg/m L,相对标准偏差（n=11）不大于1.6%。利用荧光光谱、化学发光谱对核黄素增强Na HSO3-H2O2体系化学发光的机理进行探讨,为该体系的应用研究提供了新思路。The reaction between Na HSO3 and H2O2produced ultra-weak chemiluminescence（ CL）.Riboflavin could greatly enhance the CL from Na HSO3-H2O2 system. The CL intensity was linearly increased with concentration of riboflavin in the range of 0. 02-2. 0 μg / m L. Based on the phenomenon,a high sensitive chemiluminescent method was developed for the determination of riboflavin in tablet samples. The detection limit was 0. 007 μg / m L and the relative standard deviation was not more than 1. 6%. Furthermore,fluorescent and chemiluminescent spectrum was utilized to illustrate the chemiluminescent mechanism,which would extend the application of Na HSO3-H2O2 system.

关 键 词：化学发光 亚硫酸氢钠 过氧化氢 核黄素 

分 类 号：O734[理学—晶体学] Q563.2[生物学—生物化学]