不同比例附子配黄连在Caco-2细胞模型中跨膜转运研究  

Research of Transmembrane Transport of Different Proportions of Fuzi(Aconitum carmichaeli debx.)with Huanglian(Coptis chinensis) in Caco-2 Cells Model

作　　者：周旺洋 贺秀云[2] 刘梦婷[2] 吕秋菊[2] 秦海燕[2] 李越兰[2] 宋捷民[2] 

机构地区：[1]浙江中医药大学附属第三医院,浙江杭州310005 [2]浙江中医药大学,浙江杭州310053

出　　处：《中国中医急症》2015年第5期753-756,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81073067)

摘　　要：目的通过Caco-2细胞模型,研究不同比例的附子配黄连在Caco-2细胞中主要成分的吸收和转运情况。方法利用Caco-2细胞模型和高效液相色谱法,研究附子配黄连比例为1∶1、1∶2、2∶1时的吸收和转运,计算跨膜转运量和表观渗透系数,3种比例药物配伍对吸收和转运的影响。结果附子∶黄连比例为1∶1、1∶2、2∶1时,盐酸小檗碱在150 min时的跨膜转运量AP→BL侧分别为0.625,0.161,0.055μg,BL→AP侧分别为1.308,0.178,0.064μg,表观渗透系数分别为2.095,1.107,1.172μg,次乌头碱在150 min时的跨膜转运量AP→BL侧分别为0.634,0.401,0.204μg,BL→AP侧分别为0.534,0.108,0.067μg,表观渗透系数分别为0.842,0.268,0.328。结论附子配黄连1∶1用量比优于1∶2、2∶1的用量比,证明临床用量比的最佳性。Objective： To study the absorption and transport condition of the main components of different proportions of Fuzi（Aconitum carmichaeli debx.） with Huanglian（Coptis chinensis） in Caco-2 cells model. Methods： The Caco-2 cells model and high performance liquid chromatography（HPLC） method were establish to study the absorption and transport condition when the aconite-coptis ratio was 1 ∶1,1 ∶2,2 ∶1. The transmembrane transport amount and the apparent permeability coefficients were calculated the influence of the 3 ratios of the two drugs on absorption and transport. Results： When the aconite-coptis ratio was 1 ∶1,1 ∶2,2 ∶1,respectively,the transmembrane transport amounts（μg） at 150 min of berberine hydrochloride on AP→BL side are 0.625,0.161,0.055. On BL →AP side were 1.308,0.178,0.064,and the apparent permeability coefficients were 2.095,1.107,1.172,the transmembrane transport amounts（μg） at 150 min of hypaconitine on AP →BL side were 0.634,0.401,0.204,on BL →AP side were 0.534,0.108,0.067,and the apparent permeability coefficients were 0.842,0.268,0.328. Conclusion： The aconite-coptis ratio of 1∶1 was better than the ratio of 1∶2 and 2∶1. It is proved the best clinical ratio.

关 键 词：附子配黄连 比例 CACO-2细胞 跨膜转运 

分 类 号：R289.5[医药卫生—方剂学]