高原脐贴软膏对家兔皮肤急性毒性和刺激性的实验研究  

Study of Transdermal Drug Delivery of Altiplano Navel Balm on Acute Toxicity and Irritation in Experimental Rabbits

作　　者：江晓霁[1,2] 方勇飞[2] 冯晓枫[1] 潘柏良[3] 流沙[1] 陈筱珺 王恒[2] 刘晓飞[2] 

机构地区：[1]解放军第三二四医院,重庆400020 [2]第三军医大学西南医院,重庆400038 [3]重庆市中医院,重庆400021

出　　处：《中国中医急症》2015年第5期769-770,821,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：军队中医药科研专项重点课题(2010ZYZ134)

摘　　要：目的观察高原脐贴软膏对家兔皮肤是否有急性毒性及刺激性。方法将16只家兔随机分为4组,即完整皮肤实验组、完整皮肤对照组、破损皮肤实验组和破损皮肤对照组。在脱毛及破损皮肤造模24 h后,分别予试验组和对照组家兔高原脐贴软膏和空白基质软膏。给药24 h后,连续观察14 d并称量家兔体质量。家兔12只,随机分为完整皮肤组和破损皮肤组。家兔背部两侧分别给予高原脐贴软膏和空白基质软膏,连续观察72 h有无局部皮肤刺激反应。结果试验家兔无急性中毒表现,试验组与对照组家兔体质量无明显差异(P>0.05)。家兔接触高原脐贴软膏后皮肤刺激分值为0,无皮肤刺激性。结论高原脐贴软膏经皮给药对家兔无急性毒性,对家兔皮肤无刺激性,是一种安全性较好的外用制剂。Objective： To observe the acute toxicity and dermal irritation of transdermal drug delivery（TDD） of altiplano navel balm on experimental rabbit. Methods： 1）Skin acute toxicity test：16 healthy New Zealand rabbits were randomly divided into 4 groups including the experimental group with integral skin,the control group with integral skin,the experimental group with damaged skin and control group with damaged skin. Altiplano navel balm and placebo balm were applied to the skin of rabbits respectively,and were removed after 24 hours. The observations were continued for 14 days.All rabbits were weighed at 7th day and 14 th day. 2）Skin irritation test： 12 New Zealand rabbits were randomly divided into integral skin group and damaged skin group.Atiplano navel balm and placebo balm were applied to the right or left back skin of those rabbits respectively,and removed after 24 hours. Observations were continued for 72 hours to evaluate skin irritation. Results： None of experimental rabbits was dead and poisoned. There was no significant difference between the weight of experimental groups with those of control groups in 7th day and 14 th day（P〉0.05）. At 1,24,48,72 hours after removed drug,neither erythema nor edema occurred on the integral skin or damaged skin. Conclusion： Altiplano navel balm is safe. It is non-toxic to apply skin without excitant.

关 键 词：高原脐贴软膏 皮肤急性毒性 皮肤刺激性 

分 类 号：R285.5[医药卫生—中药学]