HPLC法测定新伤止痛颗粒中盐酸小檗碱含量  

Determination of Berberine Hydrochloride Content in Xinshangzhitong Granule by HPLC

作　　者：程培秀 郑万景 李建 周小力 魏银贵 张彬 

机构地区：[1]四川省雅安市食品药品检验所,四川雅安625000 [2]四川省雅安市名山区中医医院,四川雅安625000 [3]雅安三九药业有限公司,四川雅安625000

出　　处：《中国中医急症》2015年第5期803-804,850,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：基金项目:四川省雅安市中医重点建设专科

摘　　要：目的建立高效液相色谱（HPLC）法测定新伤止痛颗粒中盐酸小檗碱含量。方法采用反相HPLC法,色谱柱为phenomenex Gamini-C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-0.033 mo L/L磷酸氢二钾溶液（38∶62）,检测波长为345 nm,流速1.0 m L/min,柱温35℃。结果盐酸小檗碱进样量在0.01184~0.1776μg范围内与峰面积呈良好的线性关系（r=0.99996）,平均加样回收率为98.01%,RSD=1.83%（n=6）。结论该方法操作简单、准确、重现性好,可用于新伤止痛颗粒中盐酸小檗碱的含量测定。Objective： To establish a method for determining berberine hydrochloride content in Xinshangzhitong Granule by HPLC. Methods： Berberine hydrochloride was analyzed by using Phenomenex Gamini-C18column（250 mm×4.6 mm,5 μm） with the mobile phase of acetonitrile-0.033 mol/L dipotassium hydrogen phosphate（38∶62） at the flow rate was 1.0 m L/min. The detection wavelength was 345 nm and the column temperature was 35℃. Results： The linear of berberine hydrochloride was in the range of 0.01184~0.1776 μg（r=0.99996）. The average recoveries were 98.01% with RSD of 1.83%（n=6）. Conclusion： The method is simple,reliable and accurate. It can be used for the determination of berberine hydrochloride content in Xinshangzhitong Granule.

关 键 词：新伤止痛颗粒 盐酸小檗碱 HPLC法 

分 类 号：R289.5[医药卫生—方剂学]