碘-131对小鼠体外胸腺细胞自发掺入~3H-TdR的影响  

Effect of ^(131)I on the Spontaneous Incorporation of Thymocytes of ~3H-TdR into mice

作　　者：郑贺[1] 王燕[1] 万书丽 东野广智[1] 孟庆勇[1] 

机构地区：[1]广东医学院检验学院,广东东莞523808

出　　处：《中国辐射卫生》2014年第6期481-483,共3页Chinese Journal of Radiological Health

基　　金：国家自然科学基金(81072238)

摘　　要：目的在检测不同剂量碘-131对小鼠胸腺细胞自发掺入3H-TdR的基础上,观察碘-131对小鼠体外胸腺细胞自发掺入3H-TdR的影响。方法给予不同剂量碘-131组的小鼠胸腺细胞同体积的3H-TdR,采用3H-TdR自发掺入法检测各组胸腺细胞每分钟计数率(CPM)。结果终浓度为5 Bq/ml的碘-131组小鼠胸腺细胞自发掺入3H-TdR的CPM值显著高于正常胸腺细胞,终浓度为5×105Bq/ml和5×106Bq/ml组小鼠胸腺细胞自发掺入3H-TdR的CPM值明显低于正常胸腺细胞。结论低剂量的碘-131对小鼠胸腺细胞自发掺入3H-TdR具有显著的刺激作用,高剂量的碘-131对小鼠胸腺细胞自发掺入3H-TdR有明显的抑制作用。Objective In the detection of different doses of 131 I on the spontaneous incorporation of ^3H- TdR in the mice thymocytes in vitro,to observe the effect of131 I on the spontaneous incorporation of ^3H- TdR into mice thymocytes. Methods The same amount of3H- TdR was given for the different spontaneous incorporation of ^3H- TdR into thymocytes. Results Experiment in vitro shows that the value of CPM of the spontaneous incorporation of ^3H- TdR into thymocytes in131I（ final concentration is 5Bq / ml） group is markedly higher than that of the normal thymocytes group. The value of CPM of the spontaneous incorporation of ^3H- TdR into thymocytes in131I（ final concentration,5 × 10^5 Bq / ml and 5 × 10^6 Bq / ml） group is lower than that of the normal thymocytes group. Conclusion Low dose of 131 I had the stimulating effect in the spontaneous incorporation of ^3H- TdR into thymocytes in vitro and large dose of 131 I can significantly inhibit the spontaneous incorporation of ^3H- TdR into thymocytes in vitro.

关 键 词：碘-131 胸腺细胞 ^3H-TDR 自发掺入 小鼠 

分 类 号：R817.9[医药卫生—影像医学与核医学]