特发性肺纤维化患者血清基质金属蛋白酶的检测及其临床意义  

Serum levels of matrix metalloproteinases in patients with idiopathic pulmonary fibrosis and their clinical value

作　　者：谢宝松[1] 姚秀娟[1] 郑冠英[1] 李鸿茹[1] 林晟[1] 洪如钧[1] 岳文香[1] 李瑞慧[1] 

机构地区：[1]福建医科大学省立临床医学院呼吸内科,福州350001

出　　处：《中华老年医学杂志》2015年第6期648-650,共3页Chinese Journal of Geriatrics

基　　金：福建省自然科学基金项目（2012J01321）

摘　　要：目的 探讨特发性肺纤维化（IPF）患者血清基质金属蛋白酶（MMPs）水平及其临床意义. 方法 IPF患者（IPF组）39例和健康体检者（对照组）36例,采集外周静脉血5 ml,采用酶联免疫吸附法检测血清MMP 1、MMP-2、MMP-9水平,同时对IPF患者进行用力肺活量（FVC）和一氧化碳弥散量（DLCO）测定,比较两组血清MMP-1、MMP-2和MMP-9水平,以及IPF组患者血清MMPs水平与肺功能指标的相关性. 结果 IPF组患者血清MMP-1、MMP-2和MMP-9中位数（范围）分别为906.30（613.50～1139.23） μg/L、9.13（5.95～11.92）μg/L和14.56（8.24～18.96）μg/L,均明显高于对照组808.38（596.59～1249.36）μg/L、7.72（5.28～10.35）μg/L和11.38（8.07～17.14）μg/L,两组血清MMP-1、MMP-2和MMP-9水平的差异有统计学意义（Z=2.397、-3.564、-4.152,P=0.017、0.000、0.000）.IPF组患者血清MMP-2水平与DLCO占预计值百分比呈负相关（r=-0.53,P=0.011）,MMP-9水平与FVC占预计值百分比、DLCO占预计值百分比均呈负相关（r=-0.49、-0.58,P=0.020、0.005）. 结论 联合检测血清MMP-1、MMP-2和MMP-9水平有助于临床诊断IPF,MMP 2和MMP-9可能成为预测IPF预后的生物标志物.Objective To investigate serum levels of matrix metalloproteinase （MMP） 1,-2 and -9 in patients with idiopathic pulmonary fibrosis （IPF）,and to assess their clinical value.Methods 39 clinically confirmed IPF cases and 36 healthy controls were recruited.A sample of 5 ml peripheral blood was collected from each individual and serum levels of MMP-1,-2 and 9 were detected by the enzyme-linked immunosorbent assay.Forced vital capacity （FVC） and diffusing capacity of carbon monoxide （DLCO） were measured in IPF patients.Serum levels of MMP 1,2 and-9 were compared between the groups.The correlations between serum levels of MMPs and lung function indicators were analyzed.Results The median serum levels of MMP 1,MMP-2 and MMP-9 were higher in the IPF group than in the control group [906.30 （613.50-1139.23） μg/L vs.808.38 （596.59 1249.36） μg/L,9.13 （5.95-11.92） μg/L vs.7.72 （5.28 10.35） μg/L,14.56 （8.24-18.96） μg/L vs.11.38 （8.07-17.14） μg/L,Z=-2.397,-3.564,-4.152,respectively,P=0.017,0.000,0.000].In patients with IPF,the serum level of MMP-2 was negatively correlated with DLCO% （r=-0.53,P=0.011） and the serum level of MMP-9 had a negative correlation with FVC% （r=-0.49,P=0.020） and DLCO% （r=0.58,P=0.005）.Conclusions Taken together,serum MMP-1,MMP-2 and MMP-9 levels may help in the clinical diagnosis of IPF.MMP 2 and MMP-9 may be important biomarkers to predict the prognosis of IPF.

关 键 词：肺纤维化 基质金属蛋白酶类 

分 类 号：R563[医药卫生—呼吸系统]