肿瘤坏死因子相关蛋白9抑制核因子κB核转位对巨噬细胞炎症因子表达的影响  

C1q/ tumor necrosis factor-related protein 9 decreases pro-inflammatory cytokines expression in macrophage cells by inhibiting nuclear factor-κB translocation

作　　者：李俊[1] 李婷婷[1] 张鹏[1] 刘天骄[1] 黄承敏[1] 高海青[1] 郭媛[1] 

机构地区：[1]山东大学齐鲁医院心内科,250012

出　　处：《中华老年医学杂志》2015年第6期664-666,共3页Chinese Journal of Geriatrics

基　　金：国家自然科学基金（NSFC：81350025）

摘　　要：目的 探究肿瘤坏死因子相关蛋白9（CTRP9）对氧化型低密度脂蛋白（ox-LDL）诱导RAW 264.7小鼠巨噬细胞抗炎作用和机制. 方法 选择RAW264.7小鼠巨噬细胞系,分为对照组（对照组）、oxLDL组、gCTRP9＋ oxLDL组.采用油红O染色鉴定泡沫细胞,免疫印迹分析检测肿瘤坏死因子（TNF）-α、单核细胞趋化蛋白（MCP-1）及胞浆、细胞核核转录因子κB （NF-κB） p65蛋白表达水平. 结果 与对照组比较,ox-LDL组巨噬泡沫细胞中MCP-1蛋白和TNF-α蛋白表达上调（P＜0.05）,与ox-LDL组比较,gCTRP9＋ oxLDL组TNF-α和MCP-1的蛋白表达降低（P＜0.05）.oxLDL组与对照组比较,NF-κB表达增加（P＜0.05）;与ox-LDL组比较,gCTRP9组2h和8hNFκB P65表达下降,1.03±0.06比0.17±0.10和0.31±0.03（均P＜0.05）. 结论 oxLDL可诱导巨噬细胞表达TNF-α和MCP-1,gCTRP9可减少oxLDL的促炎作用,NF-κB信号转导通路可能参与了上述抗炎作用机制,提示gCTRP9可能具有抗炎,抗动脉粥样硬化的保护性作用.Objective To investigate the anti-inflammatory effect of C1q/ tumor necrosis factor （TNF）-related protein 9 （CTRP9） in RAW264.7 mouse macrophage cells treated with oxidized low density lipoprotein （oxLDL）,and to explore its mechanism.Methods RAW264.7 mouse macrophage cells were divided into three groups：the control group,the oxLDL group （treated with oxLDl） and the gCTRP9-oxLDL group （pretreated with recombinant globular domain of CTRP9 and stimulated by oxLDL）.Foam cells were detected by oil red O staining.Western blot was used to detect the expressions of pro-inflammatory cytokines tumor necrosis factor-α （TNF-α） and monocyte chemoattractant protein 1 （MCP-1）.In addition,the expression levels of NF-κB p65 in cytoplasm and nucleus proteins extraction were both determined.Results The relative levels of MCP-1 and NF-κB were increased in the oxLDL group as compared with the control group （1.66±0.09 vs.1.03±0.10,0.52±0.11 vs.1.03±0.06,both P〈0.05）.The expression levels of TNF-α and MCP-1 were decreased in gCTRP9＋oxLDL group as compared with the oxLDL group （both P〈0.05）.The expression level of NF κB p65 in nucleus 2 and 8 h after treatment was lower in the gCTRP9＋oxLDL group than in the oxLDL group （1.03±0.06 vs.0.17±0.10,0.31±0.03,both P〈0.05）.Conclusions oxLDL may induce the expressions of inflammatory cytokines of TNF α and MCP-1 in macrophage ceils.gCTRP9 pretreatment could reduce the oxLDL-induced pro inflammatory effect and nuclear factor κB translocation may be involved in this process,which suggests that gCTRP9 may play a protective role in anti inflammatory and anti-atherosclerosis.

关 键 词：肿瘤坏死因子 动脉粥样硬化 

分 类 号：R543.5[医药卫生—心血管疾病]