不同培养基对Bacillus subtilis XF-1产脂肽抗生素的影响  

EFFECTS OF DIFFERENT MEDIA ON CYCLIC LIPOPEPTIDES PRODUCED BY Bacillus subtiIis XF-1

作　　者：李兴玉[1,2] 毛自朝[3] 吴毅歆[3] 何月秋[1,3] 

机构地区：[1]云南农业大学农业生物多样性应用技术国家工程中心,昆明650201 [2]云南农业大学基础与信息工程学院,昆明650201 [3]云南农业大学农学与生物技术学院,昆明650201

出　　处：《内蒙古农业大学学报（自然科学版）》2014年第6期49-53,共5页Journal of Inner Mongolia Agricultural University(Natural Science Edition)

基　　金：农业部公益性行业(农业)科研专项(201003029);云南省自然科学基金重点项目(2008CC024);云南省科技强省专项(2009EB060)

摘　　要：为探索培养基对脂肽种类及产率的影响,本文考察了Bacillus subtilis XF-1在5种培养基中发酵的生物量和丰源素、表面活性素及伊枯草菌素等环脂肽化合物产率,并通过HPLC分析了发酵液中化合物种类的变化。试验结果表明,各培养基在相同条件下生物量由大到小的顺序为MOLP(2.07 g/L)、PSA(1.28 g/L)、LB(1.03 g/L)、Landy(0.326 g/L)、蔗糖取代葡萄糖的Landy培养基(LS,0.375 g/L)。经过酸沉醇提取后获得脂肽混合物,其质量由大到小的顺序为:MOLP(0.351 g/L)、PSA(0.176 g/L)、LB(0.201 g/L)、Landy(0.501 g/L)、LS(0.517 g/L)。最适合菌体生长的是MOLP培养基,脂肽产率最高的是LS,其次是Landy培养基。5种培养基发酵液中所含环脂肽类化合物色谱图基本一致。LS比Landy提高菌体生物量15%,提高脂肽类抗生素3%,因此Landy培养基和LS培养基可作为XF-1脂肽化合物合成专用培养基。Bacillus subtilis XF- 1 was cultured in 5 media to investigate the changes of its biomass,production ability of cyclic lipopeptides family（ CLPs） such as fengycin,surfactin and iturin,and the supernatants were extracted with acid and precipitated with alcohol,and then the precipitants were detected by HPLC respectively. The biomass were 2. 07,1. 28,1. 03,0. 326 and 0. 375 g /L and the mass of CLPs were 0. 351,0. 176,0. 201,0. 501 and 0. 517 g / L in MOLP,PSA,LB,Landy and LS media,and all of the HPLC chromatograms are very similar. MOLP was the best growth medium,and LS medium derived from Landy whose glucrose is replaced with sucrose produced 15% more bacteria and 3% more CLPs than Landy did. Therefore,Landy and LS might be used as specific media for XF- 1 to produce CLPs.

关 键 词：Landy培养基 蔗糖偏好 生物量 脂肽产率 

分 类 号：S435[农业科学—农业昆虫与害虫防治]