含依诺沙星微球的改性明胶膜的制备及体外释药性研究  

Preparation of a Modified Gelatin Film with Enoxacin Microspheres and Release Behaviour in vitro

作　　者：吴宏霞[1] 林媚[2] 

机构地区：[1]福建医科大学药学院实验中心,福州350108 [2]福建医科大学药学院药物化学系,福州350108

出　　处：《福建医科大学学报》2015年第1期11-15,共5页Journal of Fujian Medical University

基　　金：福建省教育厅科技A类项目(JA13148)

摘　　要：目的制备含有依诺沙星微球的改性明胶膜,并研究其体外释药性。方法以乳化交联法制备依诺沙星明胶微球,以微球粒径、载药量、包封率为指标,应用正交试验优选微球的最佳制备工艺后,将载药微球分散到改性明胶膜中,对比普通载药膜测定其在pH 7.4磷酸盐缓冲液中的释放性。结果以最优工艺制备的依诺沙星微球外观圆整,分布均匀,平均粒径为（3.04±0.25）μm,〉80%微球粒径分布在1-6μm,微球平均载药量为（11.8±1.02）%,平均包封率为（78.24±3.18）%,载药微球膜和普通药膜中依诺沙星累积释放约80%各需96h和8h。结论依诺沙星微球制备工艺稳定可行,载药微球改性明胶膜体外释放缓慢,有望开发为具有长效抗菌效果的创伤性敷料。Objective To prepare a modified gelatin film with Enoxacin microspheres and to research its drug release in vitro. Methods Enoxacin gelatin microspheres were prepared by using emulsion crosslinking method. The optimum technology conditions were selected by orthogonal design method,with the diameter of the microballoon drug-bearing amount,encapsulation rate as the indexes. The microspheres loaded Enoxacin were dispersed into modified gelatin film. The release properties were studied in comparison with unmodified drug-loaded filmin phosphate buffered solution（pH=7.4）.Results The optimally prepared Enoxacin microspheres were shaped spherical,evenly distributed with mean diameter of（3.04±0.25）μm. The diameters of 80% of the particles were 1-6μm. The loading amount of Enoxacin was（11.8±1.02）% and the encapsulation efficiency was（78.24±3.18）%. 96 hours and 8hours were needed to reach the cumulative release of approximately 80% from microspheres film and unmodified drug film,respectively,in phosphate buffered solution. Conclusions The technology of Enoxacin microspheres was stable and feasible,and the gelatin film loaded microspheres showed sustained-release evidently in vitro. The modified gelatin film with Enoxacin microspheres has the hope of becoming a novel wound dressing with sustained-released antibacterial effect.

关 键 词：依诺沙星 微球体 明胶 膜剂 药用制剂/代谢 

分 类 号：R943[医药卫生—药剂学]