GBE对HIBD新生大鼠脑组织MMP-9及TIMP-1表达影响的研究  

Effect of Ginkgo biloba extract on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in brain of neonatal rats with ischemic brain damage

作　　者：曹巧玲[1] 王瑞华[1] 陈广斌[1] 史治宙[1] 庞国象[1] 

机构地区：[1]深圳市龙岗区人民医院儿科,广东深圳518172

出　　处：《热带医学杂志》2015年第5期614-616,645,共4页Journal of Tropical Medicine

基　　金：深圳市龙岗区科技发展基金项目(YS2012092)

摘　　要：目的通过研究银杏叶提取物(GBE)对缺氧缺血性脑损伤(HIBD)新生大鼠脑组织基质金属蛋白酶-9(MMP-9)、基质金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响,探讨GBE的脑保护作用及机制。方法 90只新生7 d SD大鼠随机分为假手术组(n=30)、HIBD模型组(n=30)、GBE治疗组(n=30)。模型制造成功后24 h、72 h、168 h三个时间点,采用干湿质量法测定大鼠脑组织含水量,RT-PCR法检测各组大鼠脑MMP-9、TIMP-1 m RNA的表达情况。结果 CBE组神经系统异常症状较HIBD组更轻。HIBD组大鼠脑组织含水量在缺氧缺血后逐渐上升,72 h达到高峰,168 h开始下降。各时间点均高于假手术组(P<0.05)。同时,MMP-9及TMP-1m RNA表达量与脑含水量呈正相关。GBE治疗组明显小于HIBD模型组(P<0.05),高于假手术组(P<0.05)。结论 GBE对HIBD脑损伤的保护作用可能与其调节MMP-9及TIMP-1的表达有关。Objective To investigate the effect of Ginkgo biloba extract on the expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinases-1 in rat’s brain after hypoxic-ischemic brain damage. Methods 7-day-old SD rats（n =90） were random ly divided into 3 groups ： sham-operation group, HIBD group and GBE treatment group. Each group was divided into three sub-groups（n=10 in each sub-group） based on different time points after HIBD（24 h, 72 h,168 h）. Changes of brain water content were determined by dry-wet weight method. RT-PCR methods were used to detect the expressions of MMP-9 and TIMP-1. Results Brain water content increased in HIBD group,peaked at 72 h,decreased at 168 h, however, the contents at each time point were higher than those of the sham-operation group（P 〈0.05）.Meanwhile,the expressions of MMP-9 and TIMP-1 m RNA were upregulated in similar fashion.They were significantly lower than those of HIBD group at each time point（P 〈0.05）. Conclusion The protective effects of Ginkgo biloba extract on nervous system of hypoxic ischemic brain damage may be through upregulating of MMP-9 and TIMP-1 expression.

关 键 词：银杏叶提取物 缺氧缺血性脑损伤 新生大鼠 MMP-9 TIMP-1 

分 类 号：R965[医药卫生—药理学]