河南地区慢性丙型肝炎患者丙型肝炎病毒基因型分布研究  

Distribution of hepatitis C virus genotypes among chronic hepatitis C patients in Henan province

作　　者：李威[1,2] 曾艳丽[1,2] 刘俊平[1,2] 宁会彬 康谊[1,2] 尚佳[1,2] 

机构地区：[1]郑州大学人民医院 [2]河南省人民医院感染性疾病科,郑州450003

出　　处：《中华实用诊断与治疗杂志》2015年第6期538-539,共2页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家自然科学基金青年科学基金(81401706);河南省医学科技攻关项目(201303120);河南省医学科技攻关普通项目(201003107);河南省医学科技攻关省部共建项目(201401015);国家临床重点专科建设项目(2013)

摘　　要：目的调查河南地区慢性丙型肝炎患者丙型肝炎病毒(hepatitis C virus,HCV)基因型的分布特点。方法 106例慢性丙型肝炎患者的血清标本,采用反转录PCR扩增HCV NS5B区,产物直接测序,并构建进化树进行HCV基因分型。结果 106例患者中HCV分型阳性95例(89.6%),共检出7种基因型:1a、1b、2a、2b、3a、3b、6a,其中1a亚型1例(1.1%)、1b亚型56例(58.9%)、2a亚型18例(18.9%)、2b亚型1例(1.1%)、3a亚型6例(6.3%)、3b亚型2例(2.1%)、6a亚型11例(11.6%)。结论河南地区慢性丙型肝炎患者HCV基因型以1b型为主,其次为2a和6a型,基因型的分布呈现多样化。Objective To investigate the current distribution of hepatitis C virus（HCV）genotypes in patients with chronic hepatitis C（CHC）in Henan province.Methods A total of 106 samples from CHC patients were collected in the study.Reverse transcription-PCR was used to amplify NS5 Bregions.HCV genotypes and their subtypes were determined by phylogenetic analysis of NS5 Bsequence.Results Among these 106 samples,95（89.6%）were successfully typed by phylogenetic analysis.Seven genotypes were detected including 1ain 1patient（1.1%）,1bin 56（58.9%）,2ain 18（18.9%）,2bin 1（1.1%）,3ain 6（6.3%）,3bin 2（2.1%）and 6ain 11（11.6%）.Conclusion The distribution of HCV genotypes indicates the diversity among CHC patients in Henan province,with a dominated prevalence of genotype1 band the higher proportion of genotype 2aand 6a.

关 键 词：丙型肝炎病毒 基因型 逆转录PCR 

分 类 号：R512.63[医药卫生—内科学]