EBV抗体与EBV DNA水平的影响因素及其相关性分析  

A study of the relationship between EBV DNA and EBV antibody levels

作　　者：陆海英[1] 魏秀琴 徐小元[1] 于岩岩[1] 吴赤红[1] 刘丹[1] 

机构地区：[1]北京大学第一医院感染疾病科,北京100034 [2]武威市人民医院,甘肃武威733300

出　　处：《临床肝胆病杂志》2015年第5期766-770,共5页Journal of Clinical Hepatology

基　　金：国家十二五科技重大专项资助项目(2013ZX10002004)

摘　　要：目的：分析 EB 病毒（EBV）抗体及 EBV DNA 的影响因素及关联性。方法回顾性分析2014年1－9月在北京大学第一医院就诊的143例患者的年龄、性别、血常规、肝功能、EBV 抗体及 DNA 检查结果，用 Spearman 相关性分析对 EBV 抗体及 EBV DNA 的相关影响因素进行分析，多个独立样本间比较使用 Kruskal －Wallis H 检验，独立样本间两两比较使用 Mann －Whitney U 检验。结果血清 EBV DNA（r ＝0．169～0．693，P ＜0．05）、EBV －VCA －IgM（r ＝0．153～0．434，P ＜0．05）均与外周血淋巴细胞 EBV DNA 、ALT、AST、ALP、GGT、白细胞（WBC）及异形淋巴细胞呈正相关，并且两者也呈正相关（r ＝0．434，P ＝0．00）；外周血淋巴细胞 EBV DNA 与血清EBV DNA、EBV －VCA －IgM、AST、ALP、WBC 及异形淋巴细胞呈正相关（r ＝0．207～0．693，P ＜0．05）。外周血淋巴细胞 EBV DNA 阳性率70．6％（101／143），血清 EBV DNA 阳性率32．2％（46／143）。EBV －VCA －IgM阳性率22．4％（32／143），22．5％（25／111）的 EBV－VCA －IgM阴性患者血清 EBV DNA 阳性。血清 EBV DNA 阳性患者 EBV －VCA －IgM阳性率（21／46，45．7％）明显高于外周血淋巴细胞 EBV DNA 阳性者（29／101，28．7％），差异有统计学意义（χ2＝7．95，P ＜0．01）。结论血清 EBV DNA 和 EBV －VCA －IgM有较强的关联性，并且前者的阳性率高于后者，更有助于 EBV 感染的早期诊断，联合检测两项指标可提高 EBV 现症感染的检出率。Objective To study the relationship between Epstein -Barr virus （EBV）DNA and EBV antibody levels.Methods A retrospec-tive study was conducted on 143 patients who underwent EBV DNA testing in serum and lymphocytes from January to August,2014.The clini-cal data,including age,sex,blood routine and biochemical examination,serum/lymphocyte EBV DNA,EBV -VCA -IgM,and EBV -VCA -IgG were recorded.Spearman correlation analysis was used to measure the degree of the relationships.Kruskal -Wallis H test or Mann -Whitney U test were applied to compare the differences between three or two groups.Results The spearman rank correlation coeffi-cient between serum EBV DNA and EBV -VCA -IgMlevels was 0.434 （P =0.00）,while the correlation coefficients of serum EBV DNA level and EBV -VCA -IgMlevel with lymphocyte EBV DNA,alanine aminotransferase （ALT）,aspartate aminotransferase （AST）,alka-line phosphatase （ALP）,gamma -glutamyl transpeptidase （GGT）,white blood cells （WBC）,and heterotypic lymphocytes ranged from 0.169 to 0.693 （P 〈0.05）and from 0.153 to 0.434 （P 〈0.05）,respectively.The spearman rank correlation coefficient of lymphocyte EBV DNA with serum EBV DNA,EBV -VCA -IgM,AST,ALP,WBC,and heterotypic lymphocytes ranged from 0.207 to 0.693 （P 〈0.05）.The positive rates of EBV DNA in lymphocytes and serum were 70.6% （101 /143）and 32.2% （46/143）,respectively.The EBV -VCA-IgMpositive rate was 22.4% （32 /143）,and 22.5% （25 /111）of the patients with negative EBV -VCA -IgMwere positive for serum EBV DNA.The overall positive rate for EBV -VCA -IgG was 91.6% （131 /143）.The EBV -VCA -IgM positive rate in patients with positive serum EBV DNA （21 /46,45.7%）was significantly higher than that in patients with positive lymphocyte EBV DNA （29 /101, 28.7%）（χ2 =7.95,P 〈0.01）.Conclusion Serum EBV DNA level is strongly correlated with EBV -VCA -IgMlevel.Moreover,ser-um EBV DNA exhibits a higher positive rate than EBV -VCA -IgM.Simultaneous detection of serum EBV DNA and EBV

关 键 词：爱泼斯坦巴尔病毒感染 EBV DNA 

分 类 号：R725.1[医药卫生—儿科]