病毒性脑炎患儿血清和脑脊液中肺表面活性物质相关蛋白D的变化  

Changes of surfactant protein-D in serum and cerebrospinal fluid in children with viral encephalitis

作　　者：王文娟[1] 王学禹[1] 

机构地区：[1]山东大学附属省立医院小儿神经科,山东济南250021

出　　处：《临床儿科杂志》2015年第6期535-538,共4页Journal of Clinical Pediatrics

摘　　要：目的探讨病毒性脑炎(VE)患儿血清和脑脊液中肺表面活性物质相关蛋白D(SP-D)的变化。方法采用酶联免疫吸附试验双抗体夹心法分别检测30例VE患儿急性期及恢复期以及12例非脑炎儿童血清和脑脊液中的SP-D,并进行比较分。结果 VE患儿急性期、恢复期以及对照组之间血清和脑脊液SP-D水平差异有统计学意义(F=103.58、118.15,P均<0.01)。其中,VE急性期、恢复期患儿的血清和脑脊液SP-D水平均低于对照组,差异有统计学意义(P<0.01);VE恢复期血清和脑脊液SP-D水平较VE急性期有所提高,差异有统计学意义(P均<0.01)。VE患儿脑脊液中SP-D水平与脑脊液有核细胞数呈负弱相关(r=-0.317,P=0.014)。结论 SP-D可能参与病毒性脑炎的发病过程,检测血清和脑脊液中的SP-D对VE诊断具有一定的价值。Objective To investigate the changes of the pulmonary surfactant protein-D（SP-D） in serum and cerebrospinal fluid in children with viral encephalitis（VE）. Methods The levels of SP-D in serum and cerebrospinal fluid were detected by a double-antibody sandwich enzyme-linked immunosorbent assay and compared in thirty children with VE in acute and convalescent phases and in 12 children without VE. Results The levels of SP-D in serum and cerebrospinal fluid between groups of VE acute phase and convalescent phase and no VE were statistically significant（F=103.58,118.15, all P〈0.01）. The levels of SP-D in serum and cerebrospinal fluid in children with VE in acute phase and in convalescent phase were significantly lower than children without VE（P〈0.01）. The levels of SP-D in serum and cerebrospinal fluid in children with VE in convalescent phase were all significantly higher than those in acute phase（P〈0.01）. In children with VE, the level of SP-D in cerebrospinal fluid was weakly correlated negatively with the count of nucleated cells. Conclusions SP-D might be involved in the pathogenesis in VE. The detection of SP-D in serum and cerebrospinal fluid has a certain value for diagnosis of VE.

关 键 词：病毒性脑炎 肺表面活性物质相关蛋白 血清 脑脊液 儿童 

分 类 号：R725.1[医药卫生—儿科]