河南省猪戊型肝炎病毒HN-JY40的全基因组序列测定及分析  

Cloning and Sequence Analyses of Genome of Swine Hepatitis E Virus(HEV)HN-JY40 Strains Isolated from Henan Province

作　　者：张小霞[1] 张亲[1] 梁振普[1] 许锋[1] 邵新峰[1] 

机构地区：[1]河南农业大学生命科学学院,郑州450002

出　　处：《病毒学报》2015年第3期231-238,共8页Chinese Journal of Virology

摘　　要：本文旨在测定猪戊型肝炎病毒HN-JY40株的全基因组序列,并对其序列特征和进化关系进行分析。设计8对引物,利用RT-PCR、3′RACE和5′RACE技术得到了戊型肝炎病毒（Hepatitis E virus,HEV）河南分离株HNJY40的近似全基因组序列,命名为＂HN-JY40＂;用Expasy、Megalign、Clustal X和MEGA 4等生物学软件进行序列分析、同源比对和进化树的构建。测序得到的HN-JY40序列去除polyA后全长7 223bp,5′UTR有9个碱基,3′UTR全长69bp。ORF1（5 124bp）编码1 707个氨基酸,ORF2（2 025bp）编码674个氨基酸,ORF3（345bp）编码114个氨基酸。全基因组相似性分析发现,HN-JY40为典型的基因4型病毒,且属于一个新亚型。猪HN-JY40ORF1的部分核苷酸（153~432bp）与人源HK104-2004同源性高达到96%,为揭示戊型肝炎的跨种传播提供了新的分子生物学依据。In the present study,the genomic sequence characteristics of HN-JY40 strains of the hepatitis E virus（HEV）isolated from pigs in Henan Province,China,were analyzed and the evolutionary relationship between HN-JY40 and other sequenced strains examined.The whole genome of HN-JY40 was sequenced and analyzed by reverse transcription-polymerase chain reaction,3′rapid amplification of cDNA ends（3′RACE）and 5′RACE.Bioinformatic analyses were carried out with Megalign,Expasy,clustal x,and MEGA 4software.The genome of HN-JY40 was 7 223 bp in size upon removal of polyA sequences.Sizes were 9bp and 69 bp at 5＇and 3＇non-coding regions,respectively.The genome of HN-JY40 was predicted to contain three open reading frames（ORFs）：ORF1（5 124bp）encoding 1 707 amino acids;ORF2（2 025bp）encoding 674 amino acids;ORF3（345bp）encoding 114 amino acids.Phylogenetic-tree analyses indicated that HN-JY40 is a typical type-IV virus that belongs to a new subgenotype of HEV genotype 4.We sequenced and analyzed the whole genome of HN-JY40.This strategy elicited the genomic characteristics of the HEV isolated from pigs in Henan Province as well as the evolutionary relationships between HNJY40 and other HEV isolates from pigs.We revealed that the ORF1 of HN-JY40（153-432nt）and human HK 104-2004 had high similarity,which offers molecular evidence for uncovering the interspecies transmission of the HEV.

关 键 词：猪戊型肝炎病毒HN-JY40 RT-PCR 3′RACE 5′RACE 序列分析 同源性 

分 类 号：S852.65[农业科学—基础兽医学]