不同浓度 hTNFα对软骨细胞及其基质相关基因表达的影响  

Effects of different concentrations of hTNFα on chondrocytes and matrix-associated gene expression

作　　者：李敏[1] 李舸[2] 贾欢欢[2] 吴玉娥[2] 唐璐[1] 陆幸妍[1] 陈珺[1] 杨国柱[1] 张钰[2] 李青南[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广州510006 [2]广东省实验动物重点实验室,广东省实验动物监测所,广州510663

出　　处：《中国骨质疏松杂志》2015年第5期541-545,共5页Chinese Journal of Osteoporosis

摘　　要：目的探讨软骨细胞在肿瘤坏死因子(TNFα)作用下对其分泌基质蛋白及相关基因mRNA表达的影响。方法体外培养的小鼠软骨细胞用25,50,100 ng/ml的h TNFα干预,不加入TNFα作为对照,通过RT-q PCR检测h TNFα对软骨细胞分泌基质蛋白及相关基因mRNA表达的影响。结果 h TNFα能降低软骨细胞分泌的Ⅱ型胶原及刺激促Ⅱ型胶原分泌的SOX9基因的表达;对基质可提高基质酶MMP-9,MMP-13,ADAMTS-4和ADAMTS-5基因的表达,提高炎性因子IL-1α,TGFβ,并增加凋亡基因BAX,caspase3的表达。结论 h TNFα通过不同途径影响软骨细胞及基质相关基因的表达,从而促进了关节软骨的降解。Objective To investigate the effect of tumor necrosis factor （ TNFα） on the secretion of matrix protein and mRNA expression of related genes in cartilage cells.Methods Mouse chondrocytes were cultured in vitro and were additioned with 25, 50, and 100 ng/ml of hTNFα.No hTNFαcultures were as control.mRNA expression of related genes in chondrocytes was detected using RT-qPCR.Results hTNFαreduced type II collagen secretion in cartilage cells and stimulated SOX9 gene expression that stimulates pro-II collagen secretion.It increased gene expression of MMP-9, MMP-13, ADAMTS-4, and ADAMTS-5 in the matrix.It also increased inflammation factors IL-1αand TGFβ, and increased apoptotic gene expression of BAX and caspase 3. Conclusion hTNFαaffects the expression of matrix-related genes in cartilage cells by different ways, thus stimulating the degradation of articular cartilage.

关 键 词：人源肿瘤坏死因子α 软骨细胞 软骨基质 基质酶 白细胞介素1Α 前凋亡蛋白 

分 类 号：R68[医药卫生—骨科学]