中医不同治法对骨质疏松症小鼠骨骼、骨骼肌Ihh含量影响的比较研究  

Comparison of the effect of treatment with different Chinese medicine on Ihh expression in bone and skeletal muscle in osteoporosis mice

作　　者：杨芳[1] 孙鑫[1] 郑洪新[1] 林庶如[1] 王拥军[2] 

机构地区：[1]辽宁中医药大学基础医学院,沈阳110847 [2]上海中医药大学脊柱病研究所上海中医药大学龙华医院,上海200032

出　　处：《中国骨质疏松杂志》2015年第5期550-552,共3页Chinese Journal of Osteoporosis

基　　金：国家自然基金青年基金(81001485);国家重点基础研究发展计划(973)计划(2010CB530401);辽宁省教育厅高校杰出青年学者成长计划(LJQ2012084)

摘　　要：目的观察不同治法对骨质疏松症小鼠骨骼、骨骼肌Ihh含量变化的影响,探讨中医防治骨质疏松症的作用机制。方法除正常组外,将OPG基因敲除小鼠随机分为5组,即模型对照组(模型组)、补肾中药组(补肾组)、健脾中药组(健脾组)、活血化瘀中药组(活血组)和对照药组(福善美组)。采用酶联免疫法测定小鼠骨骼、骨骼肌Ihh含量变化。结果 1与正常组比较,模型组小鼠骨骼肌的Ihh含量显著降低(P<0.01);与模型组比较,补肾组小鼠骨骼肌的Ihh含量明显升高(P<0.05),其次为健脾组(P<0.05)。2与正常组比较,模型组小鼠骨骼的Ihh含量显著降低(P<0.01);与模型组比较,补肾组小鼠骨骼肌的Ihh含量明显升高(P<0.01),其次为健脾组(P<0.01),活血组和福善美组升高程度也比较明显(P<0.01)。结论 1骨质疏松症的形成,可能与骨骼、骨骼肌Ihh含量异常变化有关。2补肾、健脾方法通过提高骨质疏松症大鼠的骨骼、骨骼肌Ihh的含量,对骨质疏松症具有一定的防治作用。Objective To approach the mechanism of different therapies in the treatment of osteoporosis by investigating Ihh expression in the bone and skeletal muscle in mice.Methods Except in the WT group, OPG knockout mice were divided into osteoporosis model group, invigorating kidney group, invigorating spleen group, promoting blood flow group, and Fosamax group. Enzyme-linked immunosorbent assay were performed to analyze the content of Ihh in the bone and skeletal muscle.Results （1） All the OPG-/-mice showed significantly lower Ihh expression in the skeletal muscle than mice in WT group.Ihh expression in invigorating kidney group increased significantly than in invigorating spleen group and in model control （ P 〈0.05 ） .（ 2 ） Ihh expression in bone in the model group decreased significantly comparing to that in WT group （P〈0.01）.Comparing to that in model group, Ihh expression increased the highest in invigorating kidney group （P〈0.01）, followed by that in invigorating spleen group.It also increased in promoting blood flow group and Fosamax group.Conclusion （ 1 ） Osteoporosis is related to the change of Ihh expression in bone and skeletal muscle.（ 2 ） Treatment with invigorating the kidney and invigorating spleen shows effective on osteoporosis probably by up-regulating Ihh expression in the bone and skeletal muscle.

关 键 词：骨质疏松症 IHH 骨骼肌 

分 类 号：R68[医药卫生—骨科学]