重组腺病毒Ad-GFP-C197的构建及其抗肿瘤活性研究  

Construction of Recombinant Adenovirus Ad-GFP-C197 and its Antitumor Activity

作　　者：邬贤[1] 陈嘉盛 曹莹[1] 丘玉昌[1] 庞建新[1] 

机构地区：[1]南方医科大学药学院新药评价中心,中国广东广州510515

出　　处：《生命科学研究》2015年第3期237-241,275,共6页Life Science Research

基　　金：广州市科学研究专项基金项目(2014J100195)

摘　　要：端粒酶是维持端粒长度的重要组成部分,是肿瘤发生的标志物之一。构建了能表达人端粒酶逆转录酶(h TERT)C端936-1132氨基酸片段(C197)的重组腺病毒Ad-GFP-C197,并观察其对Hela细胞增殖的影响。结果显示,Ad-GFP-C197感染Hela细胞后,采用免疫印迹法检测到C197在细胞内得到高效表达。此外,AdGFP-C197明显抑制Hela细胞的增殖,诱导细胞凋亡,并且降低Hela细胞中端粒酶活性。上述研究为进一步研究h TERT C末端功能打下基础,并为肿瘤的生物治疗提供新的思路。Telomerase plays a critical role in maintaining telomere length, and it is one of the markers of the tumors. A recombinant adenovirus vector,Ad-GFP-C197, was constructed to express intact C-terminus of human telomerase reverse transcriptase（amino acid 936-1132,C197）, and its effect on the growth of Hela cells was evaluated. Western blot assay showed C197 were expressed in Hela cells after infection with AdGFP-C197. Furthermore, Ad-GFP-C197 significantly inhibited the proliferation and promoted apoptosis of Hela cells, as well as suppressed telomerase activity in Hela cells. The results highlight the potential of C-197 in tumor therapy.

关 键 词：重组腺病毒 HELA细胞 人端粒酶逆转录酶 C197 细胞凋亡 端粒酶活性 

分 类 号：R966[医药卫生—药理学]