玉米秸秆半同步与同步糖化发酵的研究  

Semi-simultaneous/Simultaneous Saccharification and Fermentation of Corn Stover

作　　者：杨德良[1] 储秋露[1] 赖晨欢[1] 谢益晖 李鑫[1] 余世袁[1] 勇强[1] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037

出　　处：《林产化学与工业》2015年第3期55-59,共5页Chemistry and Industry of Forest Products

基　　金：国际先进林业科学技术引进项目(2012-4-18);江苏高校研究生科研创新计划(CXZZ12_0536);江苏省六大人才高峰资助项目(无编号)

摘　　要：于初始底物质量浓度100 g/L,酶解6 h、12 h分别补料50 g/L条件下,以总底物质量浓度200 g/L的绿液预处理玉米秸秆,先预酶解24 h后同步糖化发酵48 h,体系中乙醇质量浓度47.58 g/L,乙醇得率为0.42 g/g(以纤维素计,下同)。而不经预酶解直接同步糖化发酵72 h,体系中乙醇质量浓度48.57 g/L,乙醇得率为0.43 g/g。与基于补料预酶解的半同步糖化发酵相比,补料同步糖化发酵技术工艺简单,适合于高浓度底物绿液预处理玉米秸秆的生物转化。Corn stover pretreated by green liquor was hydrolyzed for 24 h at an initial substrate concentration of 100 g / L and a fed-batch of 50 g / L substrate for 6 h and 12 h,respectively,and then simultaneously saccharified and fermented for 48 h. Ethanol with a mass concentration of 47. 58 g / L and a yield with 0. 42 g / g cellulose was obtained. As the corn stover was pretreated under the same condition for 72 h,and the mass concentration and the yield of ethanol were 48. 57 g / L ethanol and 0. 43 g / g cellulose,respectively. Compared to semi-simultaneous saccharification and fermentation based on pre-hydrolysis,the simultaneous saccharification and fermentation were suitable for the bioconversion of pretreated corn stover at high substrate loading.

关 键 词：玉米秸秆 半同步糖化发酵 同步糖化发酵 乙醇 

分 类 号：TQ35[化学工程]