造纸废弃物麦草废渣高温自水解预处理对酶水解性能的影响  

Effects of Hot-compressed Water on the Enzymatic Hydrolyzability of Papermaking Waste Wheat Straw

作　　者：黄萍[1,2] 朱圆圆[1] 徐勇[1] 勇强[1] 黄立新[2] 余世袁[1] 

机构地区：[1]南京林业大学化学工程学院,江苏南京210037 [2]中国林业科学研究院林产化学工业研究所,江苏南京210042

出　　处：《林产化学与工业》2015年第3期91-96,共6页Chemistry and Industry of Forest Products

基　　金：'十二五'国家科技支撑计划资助项目(2015BAD15B09);江苏高校优势学科建设工程资助项目(无编号)

摘　　要：对造纸废弃物麦草废渣进行高温自水解预处理,并对预处理后的麦草废渣进行组成分析和酶解,以纤维素和木聚糖的酶解得率为考核指标,研究了不同自水解预处理条件对麦草废渣酶解的影响。研究表明,预处理后麦草废渣的化学组成和物理结构均发生了改变,纤维素的含量增加,比表面积增大且促进了纤维素的酶解。酶解结果显示,温度对预处理麦草废渣的酶水解得率有着极其显著的影响。以200℃自水解预处理60 min的麦草废渣为酶水解底物(质量浓度50 g/L),添加纤维素酶35 FPIU/g(以纤维素计,下同)、β-葡萄糖苷酶25 IU/g、木聚糖酶120 U/g及2 g/L聚乙二醇(PEG6000),酶解体系50 m L,于50℃酶解36 h,其纤维素和木聚糖酶解得率分别达86.29%和74.03%,是麦草废渣经170℃自水解40 min后酶解得率的2.3倍和2.8倍。Wheat straw from papermaking waste was pretreated by hot-compressed water. The test of enzymatic hydrolysis and its compositions was studied. The effects of pretreatment conditions on waste wheat straw were investigated,and the enzymatic hydrolysis yields of cellulose and xylan were examined. Results showed that chemical composition of waste wheat straw and physical structures were changed after pretreatment,and the cellulose contents and surfaces increased. This promoted the enzymatic hydrolysis of cellulose. Enzymatic hydrolysis results showed that the temperature of pretreatment had a very significant impact on the yield. Waste wheat straw pretreated at 200 ℃ for 60 min was used as the enzyme hydrolysis substrate at a concentration of 50 g / L. Under the conditions of cellulose enzyme 35 FPIU / g（ of cellulose）,β-glucose glycosides enzyme25 IU / g,xylanase enzyme 120 U / g,PEG6000 2 g / L,enzymatic hydrolysis temperature 50 ℃,and reaction time 36 h,the hydrolysis yield of cellulose and xylan reached 86. 29% and 74. 03%,respectively. They improved 2. 3 times and 2. 8 times of those pretreated by 170 ℃ for 40 min.

关 键 词：麦草废渣 高温自水解 预处理 酶解 

分 类 号：TQ35[化学工程]