芦荟大黄素对斑马鱼胚胎发育及运动行为学的毒性研究  

Toxicity of aloe-emodine to embryos growth and behavior of zebrafish

作　　者：何秋霞[1] 董贞兰 楚杰[1] 孙桂金[2] 韩利文[1] 韩健[1] 刘可春[1] 

机构地区：[1]山东省科学院生物研究所山东省生物传感器重点实验室,山东济南250014 [2]齐鲁工业大学,山东济南250353

出　　处：《山东科学》2015年第3期23-28,共6页Shandong Science

基　　金：国家自然科学基金青年基金(31400979;81202584)

摘　　要：以斑马鱼胚胎为模型,研究芦荟大黄素对胚胎发育和行为学的影响。结果显示,1.0μg/m L及以上浓度的芦荟大黄素对斑马鱼胚胎有致死作用,随着浓度的增加和作用时间延长而增强。芦荟大黄素对斑马鱼胚胎的孵化过程有阻滞作用,1.0μg/m L及以上浓度的芦荟大黄素使斑马鱼胚胎出现了卵黄延伸异常、发育迟缓和身体弯曲等畸形现象,但是对斑马鱼仔鱼的运动行为学无明显影响。本实验结果提示,临床应用芦荟大黄素治疗如孕妇这类特殊病人时,应充分考虑药物的剂量和用药时间。We addressed the impact of aloe-emodine on embryos growth and behavior of zebrafish with zebrafish embryo as a model. Experimental results show that aloe-emodineof more than 1. 0 μg/mL concentration has lethal effect to zebrafish embryos. This effect is strengthened with the increase of concentration and action time. Aloe-emodine has obstruction effect to the incubation of zebrafish embryos. Aloe-emodine of more than 1.0 μg/mL concentration makes zebrafish embryos to exhibit such malformation as yolk abnormal extension, growth retardation and body bending. However, Aloe-emodine has no obvious effect on the behavior of zebrafish larvae. Experimental results demonstrate that we should fully consider aloe-emodine dosage and medication time when we clinically apply aloe-emodine to the treatment for such special patients as pregnant women.

关 键 词：芦荟大黄素 斑马鱼 发育毒性 行为学 

分 类 号：R965.1[医药卫生—药理学] R285[医药卫生—药学]